Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated Salmonella as a vaccine

ABSTRACT

A live genetically engineered bacterium, comprising a genetically engineered construct comprising a nucleic acid sequence encoding at least one portion of a SARS-CoV-2 antigen, the live genetically engineered bacterium being adapted for administration to a human or animal and colonization of at least one tissue under non-lethal conditions. The antigen is preferably the SARS-CoV-2 spike protein. The nucleic acid sequence preferably includes an associated promoter.

CROSS REFERENCE TO RELATED APPLICATIONS

The present application is a continuation of U.S. patent application Ser. No. 15/931,669, filed May 14, 2020, now U.S. Pat. No. 10,973,908, issued Apr. 13, 2021, the entirety of which is expressly incorporated herein by reference in its entirety.

FIELD OF THE INVENTION

The present invention relates to the field of live bacterial vectors as vaccines, and more particularly to a live attenuated bacteria expressing a portion of the SARS-CoV-2 spike protein receptor binding domain, adapted for oral administration to a human without substantial morbidity and to induce an effective preventative vaccine response.

BACKGROUND OF THE INVENTION

Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It was first identified in Wuhan, China, and has since spread globally, resulting in an ongoing pandemic. As of 10 May 2020, more than 4.08 million cases have been reported across 187 countries and territories, resulting in more than 281,000 deaths. More than 1.39 million people have been infected. en.wikipedia.org/wiki/Coronavirus_disease_2019.

Common symptoms include fever, cough, fatigue, shortness of breath, and loss of smell and taste. While the majority of cases result in mild symptoms, some progress to acute respiratory distress syndrome (ARDS), multi-organ failure, septic shock, and blood clots. The time from exposure to onset of symptoms is typically around five days but may range from two to fourteen days.

The virus is believed to be spread between people during close contact, most often via small droplets produced by coughing, sneezing, and talking. It is most contagious during the first three days after the onset of symptoms, although spread may be possible before symptoms appear, or from people who do not show symptoms. The standard method of diagnosis is by real-time reverse transcription polymerase chain reaction (rRT-PCR) from a nasopharyngeal swab. Chest CT imaging may also be helpful for diagnosis in individuals where there is a high suspicion of infection based on symptoms and risk factors; however, guidelines do not recommend using it for routine screening. Chest X-ray shows a characteristic ground glass appearance.

As is common with infections, there is a delay between the moment a person is first infected and the time he or she develops symptoms. This is called the incubation period. The incubation period for COVID 19 is typically five to six days but may range from two to 14 days, although 97.5% of people who develop symptoms will do so within 11.5 days of infection.

A minority of cases do not develop noticeable symptoms at any point in time. These asymptomatic carriers tend not to get tested, and their role in transmission is not yet fully known. However, preliminary evidence suggests they may contribute to the spread of the disease.

SARS-CoV-2 is closely related to the original SARS-CoV. [6] It is thought to have a zoonotic origin. Genetic analysis has revealed that the coronavirus genetically clusters with the genus Betacoronavirus, in subgenus Sarbecovirus (lineage B) together with two bat-derived strains. It is 96% identical at the whole genome level to other bat coronavirus samples (BatCov RaTG13). In February 2020, Chinese researchers found that there is only one amino acid difference in the binding domain of the S protein between the coronaviruses from pangolins and those from humans; however, whole-genome comparison to date found that at most 92% of genetic material was shared between pangolin coronavirus and SARS-CoV-2, which is insufficient to prove pangolins to be the intermediate host. [7]

The lungs are the organs most affected by COVID 19 because the virus accesses host cells via the enzyme angiotensin-converting enzyme 2 (ACE2), which is most abundant in type II alveolar cells of the lungs. The virus uses a special surface glycoprotein called a “spike” (peplomer) to connect to ACE2 and enter the host cell. [8] The density of ACE2 in each tissue correlates with the severity of the disease in that tissue and some have suggested that decreasing ACE2 activity might be protective,[9][10] though another view is that increasing ACE2 using angiotensin II receptor blocker medications could be protective and these hypotheses need to be tested. [11] As the alveolar disease progresses, respiratory failure might develop and death may follow. [10]

The virus also affects gastrointestinal organs as ACE2 is abundantly expressed in the glandular cells of gastric, duodenal and rectal epithelium [12] as well as endothelial cells and enterocytes of the small intestine. [13]

The virus can cause acute myocardial injury and chronic damage to the cardiovascular system. [14] Rates of cardiovascular symptoms are high, owing to the systemic inflammatory response and immune system disorders during disease progression, but acute myocardial injuries may also be related to ACE2 receptors in the heart. [14] ACE2 receptors are highly expressed in the heart and are involved in heart function. [14][15]

Although SARS-COV-2 has a tropism for ACE2-expressing epithelial cells of the respiratory tract, patients with severe COVID 19 have symptoms of systemic hyperinflammation. Clinical laboratory findings of elevated IL-2, IL-7, IL-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon-γ inducible protein 10 (IP-10), monocyte chemoattractant protein 1 (MCP-1), macrophage inflammatory protein 1-α (MIP-1α), and tumour necrosis factor-α (TNF-α) indicative of cytokine release syndrome (CRS) suggest an underlying immunopathology. Additionally, people with COVID 19 and acute respiratory distress syndrome (ARDS) have classical serum biomarkers of CRS, including elevated C-reactive protein (CRP), lactate dehydrogenase (LDH), D-dimer, and ferritin. [16]

Systemic inflammation results in vasodilation, allowing inflammatory lymphocytic and monocytic infiltration of the lung and the heart. In particular, pathogenic GM-CSF-secreting T-cells were shown to correlate with the recruitment of inflammatory IL-6-secreting monocytes and severe lung pathology in COVID 19 patients. [17]

It is unknown if past infection provides effective and long-term immunity in people who recover from the disease. [18][19] Some of the infected have been reported to develop protective antibodies, so acquired immunity is presumed likely, based on the behavior of other coronaviruses. [20] However, cases in which recovery from COVID 19 was followed by positive tests for coronavirus at a later date have been reported. [21][22][23][24] These cases are believed to be lingering infection rather than reinfection,[24] or false positives due to remaining RNA fragments. [15] Some other coronaviruses circulating in people are capable of reinfection after roughly a year. [26][27]

Three vaccination strategies are generally being investigated. First, researchers aim to build a whole virus vaccine. The use of such a virus, be it inactive or dead, aims to elicit a prompt immune response of the human body to a new infection with COVID 19. A second strategy, subunit vaccines, aims to create a vaccine that sensitizes the immune system to certain subunits of the virus. In the case of SARS-CoV-2, such research focuses on the S-spike protein that helps the virus intrude the ACE2 enzyme receptor. A third strategy is that of the nucleic acid vaccines (DNA or RNA vaccines). Experimental vaccines from any of these strategies would have to be tested for safety and efficacy. [28] Antibody-dependent enhancement has been suggested as a potential challenge for vaccine development for SARS-COV-2, but this is controversial. [29]

Cytokine release syndrome (CRS) can be a complication in the later stages of severe COVID 19. There is preliminary evidence that hydroxychloroquine may have anti-cytokine storm properties. [30]

Tocilizumab has been included in treatment guidelines by China's National Health Commission after a small study was completed. [31][32] It is undergoing a phase 2 non-randomised trial at the national level in Italy after showing positive results in people with severe disease. [33][34] Combined with a serum ferritin blood test to identify cytokine storms, it is meant to counter such developments, which are thought to be the cause of death in some affected people. [35][36][37] The interleukin-6 receptor antagonist was approved by the FDA to undergo a phase III clinical trial assessing the medication's impact on COVID 19 based on retrospective case studies for the treatment of steroid-refractory cytokine release syndrome induced by a different cause, CAR T cell therapy, in 2017. [38] Lenzilumab, an anti-GM-CSF monoclonal antibody, is protective in murine models for CAR T cell-induced CRS and neurotoxicity and is a viable therapeutic option due to the observed increase of pathogenic GM-CSF secreting T-cells in hospitalized patients with COVID 19. [39]

The Feinstein Institute of Northwell Health announced in March a study on “a human antibody that may prevent the activity” of IL-6. [40]

Transferring purified and concentrated antibodies produced by the immune systems of those who have recovered from COVID 19 to people who need them is being investigated as a non-vaccine method of passive immunization. [41] This strategy was tried for SARS with inconclusive results. [41] Viral neutralization is the anticipated mechanism of action by which passive antibody therapy can mediate defense against SARS-CoV-2. Other mechanisms, however, such as antibody-dependent cellular cytotoxicity and/or phagocytosis, may be possible. [41] Other forms of passive antibody therapy, for example, using manufactured monoclonal antibodies, are in development. [41] Production of convalescent serum, which consists of the liquid portion of the blood from recovered patients and contains antibodies specific to this virus, could be increased for quicker deployment. [42] The world experienced the outbreaks of coronavirus infection that threaten global pandemic in 2002-2003 by Severe Acute Respiratory Syndrome (SARS) and in 2011 by Middle East Respiratory Syndrome (MERS). In both cases, the causative agents (SARS-CoV and MERS-CoV, respectively) were newly identified coronavirus in the genus Betacoronavirus with zoonotic origin. At the end of 2019, outbreak of another coronavirus that causes respiratory-related illness was reported in Wuhan, Hubei, China, a disease now officially called “the Corona Virus Disease 2019; COVID-19”. The coronavirus that is the causative agent of this respiratory disease was identified and its genome is fully sequenced. [42] The genomic sequence of SARS-CoV-2 showed similar, but distinct genome composition of SARS-CoV and MERS-CoV. Since its first reported case in late 2019, the infection has spread to other regions in China and other countries, and the transmission rate, the mortality rate and the clinical manifestation slowly emerged. However, it will take months and may be years until we will fully grasp the whole picture of the characteristics of the pathogens and its likely origin, symptoms and the host immune responses to combat the infection. [83]

Identification of SARS-CoV-2 tropism is also warranted. In agreement with genome similarity with SARS, analysis of nucleic acid sequence within the spike protein receptor-binding domain (RBD) has been predicted that SAR-CoV-2 might also use angiotensin-converting enzyme 2 (ACE2) as a cell receptor. [43] The study performed in vitro experiments which could confirm that SAR-CoV-2 used ACE2 for cellular entry. [44] Because wild range of animal species (except rat and mouse) express ACE2, it could support the observed cross-species and human-to-human transmission events.

Currently, only limited information is available on the host innate immune status of SARS-CoV-2 infected patients. In one report where 99 cases in Wuhan were investigated, increased total neutrophils (38%), reduced total lymphocytes (35%), increased serum IL-6 (52%) and increased c-reactive protein (84%) were observed. [45] In a separate report also from Wuhan, it revealed that in 41 patients, increased total neutrophils, decreased total lymphocytes in patients of ICU vs. non-ICU care were found to be statistically different. Increased neutrophils and decreased lymphocytes also correlate with disease severity and death. Furthermore, patients needing ICU care had higher plasma levels of many innate cytokines, IP-10, MCP-1, MIP-1A, and TNFα. [46] These clinical features suggested the likelihood of involvement of highly pro-inflammatory condition in the disease progression and severity. This early high rise in the serum levels of pro-inflammatory cytokines were also observed in SARS-CoV and MERS-CoV infection, suggesting a potential similar cytokine storm-mediated disease severity. [47] [48]

Effective innate immune response against viral infection relies heavily on the interferon (IFN) type I responses and its downstream cascade that culminates in controlling viral replication and induction of effective adaptive immune response. While SARS-CoV and SARS-CoV-2 seem to share the entry receptor of ACE2, MERS-CoV uses dipeptidyl peptidase (DPP)-4 as a specific receptor. [45] The putative receptor of SARS-CoV-2, ACE2, is mainly expressed in a small subset of cells in the lung called type 2 alveolar cells.26 It has been reported that SARS-CoV directly infects macrophages and T cells, a key feature in SARS-CoV-mediated pathogenesis. [49] Whether SARS-CoV-2 infects any immune cells are still unknown. Only minimal percentages of monocytes/macrophages in the lung expressed ACE2. [50] If ACE2 is minimally expressed in the potential target immune cells, it is possible that other receptors may exist, or other cellular entry mode is utilized such as antibody-dependent enhancement.

To mount an antiviral response, innate immune cells need to recognize the invasion of the virus, often by pathogen-associated molecular patterns (PAMPs). For RNA virus such as coronavirus, it is known that PAMPs in the form of viral genomic RNA or the intermediates during viral replication including dsRNA, are recognized by either the endosomal RNA receptors, TLR3 and TLR7 and the cytosolic RNA sensor, RIG-I/MDA5. This recognition event leads to activation of the downstream signaling cascade, i.e., NF-κB and IRF3, accompanied by their nuclear translocation. In the nuclei, these transcription factors induce expression of type I IFN and other pro-inflammatory cytokines and these initial responses comprise the first line defense against viral infection at the entry site. [51] Type I IFN via IFNAR, in turn, activates the JAK-STAT pathway, where JAK1 and TYK2 kinases phosphorylate STAT1 and STAT2. STAT1/2 form a complex with IRF9, and together they move into the nucleus to initiate the transcription of IFN-stimulated genes (ISGs) under the control of IFN-stimulated response element (ISRE) containing promoters. A successful mounting of this type I IFN response should be able to suppress viral replication and dissemination at an early stage.

For SARS-CoV and MERS-CoV, the response to viral infection by type I IFN is suppressed. Both coronaviruses employ multiple strategies to interfere with the signaling leading to type I IFN production and/or the signaling downstream of IFNAR. This dampening strategy is closely associated with the disease severity. At the step of type I IFN induction, SARS-CoV interferes with the signaling downstream of RNA sensors directly or indirectly such as ubiquitination and degradation of RNA sensor adaptor molecules MAVS and TRAF3/6 and inhibiting IRF3 nuclear translocation. [52] MERS-CoV also utilizes some of these strategies with additional mechanism such as repressive histone modification. Once type I IFN is secreted, these two viruses are equipped with mechanism that inhibit IFN signaling such as decreasing STAT1 phosphorylation. The viral proteins involved in the modulation of this host type I IFN response are both structural proteins (such as M, N) and non-structural proteins (ORF proteins).

Based on the genomic sequence comparison, SARS-CoV shares overall genomic similarity with SARS-CoV or MERS-CoV, approximately 79% and 50%, respectively. The genome of SARS-CoV-2 also contains additional gene regions (10b, 13, 14). In addition, the amino acid sequences of some putative proteins of SARS-CoV-2 show only 68% similarity with that of SARS-CoV. Therefore, careful sequence comparison of each gene region may yield better prediction as how SARS-CoV-2 interferes with host innate immune response. It is partially speculative that SARS-CoV-2 utilizes similar strategies to modulate the host innate immune response, especially in dampening the type I IFN response but additional novel mechanisms may be uncovered.

Aerosolized uptake of SARS-CoV-2 leads to infection of ACE2 expressing target cells such as alveolar type 2 cells or other unknown target cells. Virus may dampen anti-viral IFN responses resulting in uncontrolled viral replication. The influx of neutrophils and monocytes/macrophages results in hyperproduction of pro-inflammatory cytokines. The immunopathology of lung may be the result of the “cytokine storms”. Specific Th1/Th17 may be activated and contributes to exacerbate inflammatory responses. B cells/plasma cells produce SARS-CoV-2 specific antibodies that may help neutralize viruses. The question marks indicated events that are still speculative or unknown.

In the severe or lethal cases of SARS-CoV or MERS-CoV infection, increased neutrophil and monocyte-macrophages influx are consistently observed. [53][54] In a mouse model of SARS-CoV infection, dysregulated type I IFN and inflammatory monocyte-macrophages are the main cause of lethal pneumonia. Therefore, excessive type I IFN with the infiltrated myeloid cells are the main cause of lung dysfunction and negatively impact the outcome of the infection. It is speculated that upon SARS-CoV or MERS-CoV infection, delayed type I IFN compromises the early viral control, leading to influx of hyperinflammatory neutrophils and monocytes-macrophages. The increases in these innate immune cells yields deteriorating consequences to infected host that manifested in lung immunopathology, including pneumonia or acute respiratory distress syndrome. In SARS-CoV-2 infection, similar scenario is expected with varying degree of immune interference. Interestingly, transmission of virus is reported to occur even in asymptomatic infected individuals. This may be indicative of delayed early response of the innate immune response.

Based on the accumulated data for previous coronavirus infection, innate immune response plays crucial role in protective or destructive responses and may open a window for immune intervention. Active viral replication later results in hyperproduction type I IFN and influx of neutrophils and macrophages which are the major sources of pro-inflammatory cytokines. With similar changes in total neutrophils and lymphocytes during COVID19, SARS-CoV-2 probably induces delayed type I IFN and loss of viral control in an early phase of infection. Individuals susceptible to CoVID19 are those with underlying diseases, including diabetes, hypertension, and cardiovascular disease. In addition, no severe cases were reported in young children, when innate immune response is highly effective. These facts strongly indicate that innate immune response is a critical factor for disease outcome.

Based on the assumption that innate immunity plays a key role, several interventions can be proposed. Type I IFN, antagonists of some key pro-inflammatory cytokines and anti-viral agents are some of these examples. When using type I IFN for treatment, in a mouse model of either SARS-CoV or MERS-CoV infection, the timing of administration is key to yield protective response. [55]

In general, the Th1 type immune response plays a dominant role in an adaptive immunity to viral infections. Cytokine microenvironment generated by antigen presenting cells dictate the direction of T cell responses. Helper T cells orchestrate the overall adaptive response, while cytotoxic T cells are essential in killing of viral infected cells. Humoral immune response, especially production of neutralizing antibody, plays a protective role by limiting infection at later phase and prevents reinfection in the future. In SARS-CoV, both T and B cell epitopes were extensively mapped for the structural proteins, S, N, M and E protein. [56]

SARS-CoV infection induces seroconversion as early as day 4 after onset of disease and was found in most patients by 14 days. Long lasting specific IgG and neutralizing antibody are reported as long as 2 years after infection. [57] For MERS-CoV infection, seroconversion is seen at the second or third week of disease onset. For both types of coronavirus infections, delayed and weak antibody response are associated with severe outcome. [56] A limited serology details of SARS-CoV-2 was reported. In a preliminary study, one patient showed peak specific IgM at day 9 after disease onset and the switching to IgG by week 2.25 Interestingly, sera from 5 patients of confirmed COVID-19 show some cross-reactivity with SARS-CoV, but not other coronavirus. Furthermore, all sera from patients were able to neutralize SARS-CoV-2 in an in vitro plaque assay, suggesting a possible successful mounting of the humoral responses.

T cell response in SARS-CoV was extensively investigated. In one study using 128 convalescent samples, it was reported that CD8+ T cell responses were more frequent with greater magnitude than CD4+ T cell responses. Furthermore, the virus-specific T cells from the severe group tended to be a central memory phenotype with a significantly higher frequency of polyfunctional CD4+ T cells (IFNγ, TNFα, and IL-2) and CD8+ T cells (IFNγ, TNFα and degranulated state), as compared with the mild-moderate group. Strong T cell responses correlated significantly with higher neutralizing antibody while more serum Th2 cytokines (IL-4, IL-5, IL-10) were detected in the fatal group. [58] For the epitope mapping, most responses (70%) were found against the structural proteins (spike, envelope, membrane, and nucleocapsid). In MERS-CoV infection, early rise of CD8+ T cells correlates with disease severity and at the convalescent phase, dominant Th1 type helper T cells are observed. [59] In an animal model, airway memory CD4+ T cells specific for conserved epitope are protective against lethal challenge and can cross react with SARS-CoV and MERSCoV. [60] As neutrophils play a destructive role in all infections, the protective or destructive role of Th17 in human coronavirus infection remains unanswered.

Current evidences strongly indicated that Th1 type response is a key for successful control of SARS-CoV and MERS-CoV and probably true for SARS-CoV-2 as well. CD8+ T cell response, even though crucial, needs to be well controlled in order not to cause lung pathology. Because most epitopes identified for both viruses concentrate on the viral structural proteins, it will be informative to map those epitopes identified with SARS-CoV/MERS-CoV with those of SARS-CoV-2. If overlapping epitopes among the three viruses can be identified, it will be beneficial for application in passive immunization using convalescent serum from recovered SARS or MERS patients. For T cell epitopes, it will help in designing cross reactive vaccine that protect against all three human coronaviruses in the future.

Current observations indicate that coronaviruses are particularly adapted to evade immune detection and dampen human immune responses. This partly explains why they tend to have a longer incubation period, 2-11 days on average compared to influenza, 1-4 days. The longer incubation period is probably due to their immune evasion properties, efficiently escaping host immune detection at the early stage of infection. As a member of the Betacoronavirus genus, immune evasion mechanism is potentially similar to those of SARS-CoV and MERS-CoV. The mechanisms of how SARS-CoV and MERS-CoV modulate host immune responses were extensively reviewed and discussed. [61][62] In brief, most mechanisms rely on the inhibition of innate immune responses, especially type I interferon recognition and signaling. The viral proteins including membrane (M) or nonstructural (NS) proteins (eg. NS4a, NS4b, NS15) are the key molecules in host immune modulation. In agreement with the aforementioned study, analysis of two MERS-CoV-infected individuals with different severity found that the type I interferon response in the poor outcome (death) patient was remarkably lower than the recovered patient. [63] For adaptive immune evasion, antigen presentation via MHC class I and MHC class II was downregulated when the macrophages or dendritic cells were infected with MERS-CoV, which would markedly diminish T cells activation. [61]

Coronaviruses interfere with multiple steps during initial innate immune response, including RNA sensing, signaling pathway of type I IFN production, and STAT1/2 activation downstream of IFN/IFNAR. This delayed or dampening type I IFN responses impinge upon adaptive immune activation. Prolonged viral persistence exacerbates inflammatory responses that may lead to immune exhaustion and immune suppression as a feedback regulatory mechanism. Biased Th2 type response also favors poor outcome of the disease.

Due to the rapid increase of SAR-CoV2 infections and affected countries, efforts toward developing an effective SARCoV2 vaccine have been ignited in many countries. By gaining knowledge from SARS and MERS vaccines development path, several research groups have been able to start SAR-CoV2 vaccine development within only a few weeks after the outbreak. The target antigen selection and vaccine platform are probably based on SARS-CoV and MERS-CoV vaccine studies. Full-length spike (S) or Si which contains receptor binding domain (RDB) might be considered as a good vaccine antigen because it could induce neutralizing antibodies that prevent host cell attachment and infection. [64][65][66] A nucleic acid-based vaccine, a DNA vaccine, showed the most advance platform in response to emerging pathogens. Moreover, during Zika virus outbreak, DNA vaccine was the first vaccine candidate that entered clinical trial (NCT02809443) (less than 1-year after the outbreak). According to the current technological advancement, mRNA vaccine, another nucleic acid-based vaccine, has been considered as disruptive vaccine technology. Recent mRNA vaccine designs have improved stability and protein translation efficiency thus it could induce robust immune responses. [67][68] Delivery system such as lipid nanoparticle, LNP was also well-optimized. [69]

By looking at the similarities and differences between the current SARS-CoV-2 and the previous outbreak of SARS and MERS, a striking similarity emerges with some unique features of its own. As the COVID-19 causes serious public health concerns across Asia and on the blink to affect world population, investigation into the characteristics of SARS-CoV-2, its interaction with the host immune responses may help provide a clearer picture of how the pathogen causes diseases in some individuals while most infected people only show mild or no symptoms at all. In addition, the study of the immune correlates of protection and the long-term immune memory from convalescent individuals may help in design prophylactic and therapeutic measures for future outbreak of similar coronaviruses.

SARS-CoV-2 has a genome size of ˜30 kilobases which, like other coronaviruses, encodes for multiple structural and non-structural proteins. The structural proteins include the spike (S) protein, the envelope (E) protein, the membrane (M) protein, and the nucleocapsid (N) protein. With SARS-CoV-2 being discovered very recently, there is currently a lack of immunological information available about the virus (e.g., information about immunogenic epitopes eliciting antibody or T cell responses). Preliminary studies suggest that SARS-CoV-2 is quite similar to SARS-CoV based on the full-length genome phylogenetic analysis [70][71], and the putatively similar cell entry mechanism and human cell receptor usage [70][72][73]. Due to this apparent similarity between the two viruses, previous research that has provided an understanding of protective immune responses against SARS-CoV may potentially be leveraged to aid vaccine development for SARS-CoV-2. [84]

Various reports related to SARS-CoV suggest a protective role of both humoral and cell-mediated immune responses. For the former case, antibody responses generated against the S protein, the most exposed protein of SARS-CoV, have been shown to protect from infection in mouse models [70][71][72]. In addition, multiple studies have shown that antibodies generated against the N protein of SARS-CoV, a highly immunogenic and abundantly expressed protein during infection [73], were particularly prevalent in SARS-CoV-infected patients [74][75]. While being effective, the antibody response was found to be short-lived in convalescent SARS-CoV patients [21]. In contrast, T cell responses have been shown to provide long-term protection [76][77][78], even up to 11 years post-infection [79], and thus have also attracted interest for a prospective vaccine against SARS-CoV [reviewed in [80]]. Among all SARS-CoV proteins, T cell responses against the structural proteins have been found to be the most immunogenic in peripheral blood mononuclear cells of convalescent SARS-CoV patients as compared to the non-structural proteins [81]. Further, of the structural proteins, T cell responses against the S and N proteins have been reported to be the most dominant and long-lasting [82].

The availability of the highly attenuated Salmonella enterica Typhimurium strain YS1646 that had been used in a phase 1 clinical cancer trial at doses up to 3×10⁸ IV was attractive for many reasons. Although S. enterica species replicate in a membrane-bound host cell compartment or vacuole [85], foreign protein antigens can be efficiently exported from the vacuole into the cytoplasm using the organism's Type 3 Secretion Signal (T3SS). Like all Salmonella enterica species, YS1646 has two distinct T3SS located in Salmonella pathogenicity islands 1 and 2 (SPI-I and SPI-II) [86] that are active at different phases of infection [87]. The SPI-I T3SS translocates proteins upon first contact of the bacterium with epithelium cells through to the stage of early cell invasion while SPI-II expression is induced once the bacterium has been phagocytosed [88]. These T3SS have been used by many groups to deliver heterologous antigens in Salmonella-based vaccine development programs [89, 90, reviewed by 91].

In recent years, live attenuated Salmonella has been increasingly used to express foreign antigens against infectious diseases and cancers. [92][93][94][95][96][97][98][99][101][102][103][104][105][106][107][108][109]

Salmonella enterica is a facultative intracellular pathogen that replicates in a unique membrane-bound host cell compartment, the Salmonella-containing vacuole [85]. Although this location limits exposure of both Salmonella and foreign proteins produced by the bacterium to the immune system, the organism's type III secretion systems (T3SS) can be exploited to translocate heterologous antigens into the host cell cytoplasm. Salmonella enterica encodes two distinct T3SS within the Salmonella pathogenicity islands 1 and 2 (SPI-I and SPI-II) that become active at different phases of infection [87]. The SPI-I T3SS translocates effector proteins upon first contact of the bacterium with epithelium cells through to the stage of early cell invasion. In contrast, SPI-II expression is induced when the bacterium has been phagocytosed. Several effector proteins translocated by these T3SSs have been tested in the promotion of heterologous antigen expression in Salmonella-based vaccine development programs but how effector protein-mediated secretion of heterologous antigens affects immune responses is still poorly understood. [89][111]

There is considerable experience in using the attenuated S. typhi vaccine strain (Ty21a: Vivotif™) in the delivery of heterologous antigens. [89] However, S. typhimurium YS1646 was selected as a candidate vector. This strain is attenuated by mutations in its msbB (LPS) and purl (purine biosynthesis pathway) genes and was originally developed as a non-specific ‘cancer vaccine’ for solid tumors. With a major investment from Vion Inc., YS1646 was carried through pre-clinical and toxicity testing in rodents, dogs and non-human primates before a phase I clinical trial where it ultimately failed [94]. More recently, YS1646 has been used to express a chimeric Schistosoma japonicum antigen that was tested in a murine model of schistosomiasis [112]. Repeated oral administration of one of the engineered strains in this study elicited a strong systemic IgG antibody response, induced antigen-specific T cells and provided up to 75% protection against S. japonicum challenge.

The present technology, according to various embodiments, consists of known and/or antigens, chimeric proteins, or combinations of proteins, that are expressed, secreted, surface displayed and/or released by bacteria and result in immunologic activity, and may optionally include the combination with secreted protease inhibitors. The bacterial delivery vector may be attenuated, non-pathogenic, low pathogenic (including wild type), or a probiotic bacterium. The bacteria are introduced either systemically (e.g., parenteral, intravenous (IV), intramuscular (IM), intralymphatic (IL), intradermal (ID), subcutaneously (sub-q), local-regionally (e.g., intralesionally, intratumorally (IT), intraperitoneally (IP), topically, intrathecally (intrathecal), by inhaler or nasal spray) or to the mucosal system through oral, nasal, pulmonary intravessically, enema or suppository administration where they are able to undergo limited replication, express, surface display, secrete and/or release the anti-cancer inhibitory proteins or a combination thereof, and thereby provide a therapeutic or preventive benefit.

Promoters, i.e., genetic regulatory elements that control the expression of the genes encoding the therapeutic molecules described above that are useful in the present technology, according to various embodiments, include constitutive and inducible promoters. A preferred constitutive promoter is that from the vector pTrc99a (Promega). Preferred inducible promoters include the tetracycline inducible promoter (TET promoter), colicin promoters, sulA promoters and hypoxic-inducible promoters including but not limited to the PepT promoter (Bermudes et al., WO 01/25397), the arabinose inducible promoter (AraBAD) (Lossner et al., 2007, Cell Microbiol. 9: 1529-1537; WO/2006/048344) the salicylate (aspirin) derivatives inducible promoter (Royo et al., 2007, Nature Methods 4: 937-942; WO/2005/054477), or a quorum-sensing (autoinduction) promoter Anerson et al., 2006 Environmentally controlled invasion of cancer cells by engineered bacteria, J. Mol. Biol. 355: 619-627.

A single promoter may be used to drive the expression of more than one gene, such as an antigen and a protease inhibitor. The genes may be part of a single synthetic operon (polycistronic), or may be separate, monocystronic constructs, with separate individual promoters of the same type used to drive the expression of their respective genes. The promoters may also be of different types, with different genes expressed by different constitutive or inducible promoters. Use of two separate inducible promoters for more than one antigen or other effector type peptide allows, when sufficient tetracycline, arabinose or salicylic acid is administered following administration of the bacterial vector, their expression to occur simultaneously, sequentially, or alternatingly (i.e., repeated). An inducible promoter is not required, and a constitutive promoter may be employed.

The present technology, according to various embodiments, consists of known and/or antigens, chimeric proteins, or combinations of proteins, that are expressed, secreted, surface displayed and/or released by bacteria and result in immunologic activity, and may optionally include the combination with secreted protease inhibitors. The bacterial delivery vector may be attenuated, non-pathogenic, low pathogenic (including wild type), or a probiotic bacterium. The bacteria are introduced either systemically (e.g., parenteral, intravenous (IV), intramuscular (IM), intralymphatic (IL), intradermal (ID), subcutaneously (sub-q), local-regionally (e.g., intralesionally, intratumorally (IT), intraperitoneally (IP), topically, intrathecally (intrathecal), by inhaler or nasal spray) or to the mucosal system through oral, nasal, pulmonary intravessically, enema or suppository administration where they are able to undergo limited replication, express, surface display, secrete and/or release the anti-cancer inhibitory proteins or a combination thereof, and thereby provide a therapeutic or preventive benefit.

The T3SS secretion system is discussed in U.S. 2019/0055569, 2010/0120124, 2012/0021517, 2015/0359909, U.S. Pat. Nos. 9,951,340, 6,306,387, expressly incorporated herein by reference.

Some bacterial pathogens comprise a type three secretion system (T3SS), which serves as a needle-like system for delivering bacterial polypeptides (effectors) into host cells. These effector polypeptides typically contribute to the virulence of the bacterial cell. In contrast, commensal microbes have not been described to comprise a T3SS.

A T3SS is a multi-protein structure found in gram negative bacteria. It moves polypeptides from the cytoplasm of the bacterial cell through the interior of the T3SS “needle” into the cytoplasm of a target cell. T3SS's are found in pathogenic strains and have been observed in pathogenic isolates of, e.g., Shigella, Salmonella, E. coli, Burkholderia, Yersinia, Chlamydia, Pseudomonas, Erwinia, Ralstonia, Rhizobium, Vibrio, and Xanthamonas. Further discussion of T3SS's can be found, e.g., in Izore et al. Structure 2011 19:603-612; Korotkov et al. Nature Reviews Microbiology 2012 10:336-351; Wooldridge, K. (ed) Bacterial Secreted Proteins. Caster Academic Press 2009; Snyder and Champness (eds.) Molecular Genetics of Bacteria. 3rd Ed. ASM Press: 2007; each of which is incorporated by reference herein in its entirety.

The suite of T3SS-related proteins in a given wild-type cell is typically divided into structural proteins (those proteins which form the needle itself), substrate proteins (those proteins which are transported through the needle to the host), and chaperones (those proteins that bind effectors in the cytoplasm to protect, process, and/or shuttle the effectors to the needle). As used herein, a “functional T3SS” refers, minimally, to the set of structural proteins which are required in order to transfer at least one polypeptide to a target cell. In some embodiments, a functional T3SS system can comprise one or more chaperone proteins. In some embodiments, a functional T3SS can comprise one or more, for example, two, three, or four, substrates which are not virulence factor (e.g., certain translocators). In some embodiments, a functional T3SS does not comprise a virulence factor which is delivered to the target cell.

As used herein, a “virulence factor” refers to those substrates which affect and/or manipulate a target cell in a manner which is beneficial to infection and deleterious to the target cell, i.e., they perturb the normal function of the target cell. Examples of actions of virulence factors include, but are not limited to, modulation of actin polymerization, induction of apoptosis, modulation of the cell cycle, modulation of gene transcription. Not all substrates are necessarily virulence factors. By way of non-limiting example, a T3SS (and a functional T3SS) can comprise proteins referred to as translocators. These substrates are secreted by the T3SS as it nears a complete form and create a pore in the target cell membrane, allowing further substrates to be delivered into the cytoplasm of the target cell, i.e., translocators are substrates in that they travel through the needle to the target cell and are also structural proteins in that they form part of the structure through which other substrates are delivered into the target cell. In some embodiments, a single polypeptide can be both a translocator and a virulence factor (e.g. IpaB of Shigella). A functional T3SS system can be introduced into a non-pathogenic bacterial cell.

Homologs of any given polypeptide or nucleic acid sequence can be found using, e.g., BLAST programs (freely available on the world wide web at blast.ncbi.nlm.nih.gov/), e.g. by searching freely available databases of sequence for homologous sequences, or by querying those databases for annotations indicating a homolog (e.g. search strings that comprise a gene name or describe the activity of a gene). The homologous amino acid or DNA sequence can be at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or more, identical to a reference sequence. The degree of homology (percent identity) between a reference and a second sequence can be determined, for example, by comparing the two sequences using freely available computer programs commonly employed for this purpose on the world wide web.

Examples of T3SS secretion signals and chaperone-binding domains are known in the art, see, e.g., Schmitz et al. Nat Methods 2009 6:500-2; which described the signals and domains of Shigella effectors and which is incorporated by reference herein in its entirety. Additional examples are known in the art, e.g., Sory et al. PNAS 1995 92:11998-20002; which is incorporated by reference herein in its entirety. It is contemplated that a T3SS signal may reduce the activity of the non-T3SS signal portion of the T3SS-compatible polypeptide once it is delivered to the target cell. Accordingly, in some embodiments, the T3SS-compatible polypeptide can comprise a cleavage site after the T3SS signal sequence. In some embodiments, the cleavage site is a site recognized by an endogenous component of the target cell, e.g., a calpain, sumo, and/or furin cleavage site. In some embodiments, instead of a cleavage site, the T3SS-compatible polypeptide can comprise a ubiquitin molecule after the T3SS signal sequence such that the ubiquitin molecule and the sequence N-terminal of it is removed from the remainder of the polypeptide by a eukaryotic target cell. In some embodiments, the first amino acid C-terminal of the ubiquitin molecule can be a methionine.

The T3SS-compatible polypeptide may be an antigen. An engineered microbial cell comprising a T3SS-compatible antigen polypeptide may be to a subject, e.g., orally.

In one aspect, described herein is a kit comprising an engineered microbial cell as described herein. In one aspect, described herein is a kit comprising an engineered microbial cell comprising a first nucleic acid sequence comprising genes encoding a functional type three secretion system (T3SS); and a second nucleic acid sequence encoding an T3SS-compatible polypeptide; wherein the engineered microbial cell is non-pathogenic with respect to a target cell. Citation or identification of any reference herein, in any section of this application, shall not be construed as an admission that such reference is available as prior art to the present application. The disclosures of each reference disclosed herein, whether U.S. or foreign patent literature, or non-patent literature, are hereby incorporated by reference in their entirety in this application, and shall be treated as if the entirety thereof forms a part of this application.

Such references are provided for their disclosure of technologies to enable practice of the present invention, to provide basis for claim language, to make clear applicant's possession of the invention with respect to the various aggregates, combinations, and subcombinations of the respective disclosures or portions thereof (within a particular reference or across multiple references). The citation of references is intended to be part of the disclosure of the invention, and not merely supplementary background information. The incorporation by reference does not extend to teachings which are inconsistent with the invention as expressly described herein, and is evidence of a proper interpretation by persons of ordinary skill in the art of the terms, phrase and concepts discussed herein, without being limiting as the sole interpretation available.

Genetically-engineered bacterial vectors represent a promising method of therapy for various diseases and as a biomolecule delivery system.

Tumor-targeted bacteria, especially those derived from wild type samples, are typically capable of producing a chronic infection without strong acute response. That is, these bacteria seem to have evolved to avoid triggering a debilitating immune response in the host while at the same time establishing long term colonization of tissues, in the case of tumor targeting bacteria, tissues which may include necrotic regions. According to some evolutionary theories, the attenuated host response to these bacteria may result from a survival benefit for the host in permitting the colonization. Indeed, there are at least anecdotal reports of successful eradication of tumors by bacterial therapy. This implies that bacteria derived from these strains can be pharmaceutically acceptable, for administration through various routes of administration.

Much research has been performed on bacterial therapies and bacterial delivery vectors. For example, tumor targeting bacteria offer tremendous potential advantages for the treatment of solid tumors, including the targeting from a distant inoculation site and the ability to express therapeutic agents directly within the tumor (Pawelek et al., 1997, Tumor-targeted Salmonella as a novel anticancer agent, Cancer Research 57: 4537-4544; Low et al., 1999, Lipid A mutant salmonella with suppressed virulence and TNF-alpha induction retain tumor-targeting in vivo, Nature Biotechnol. 17: 37-41). However, the primary shortcoming of tumor-targeted bacteria investigated in the human clinical trials (Salmonella strain VNP20009 also known as YS1646, and its derivative TAPET-CD; Toso et al., 2002, Phase I study of the intravenous administration of attenuated Salmonella typhimurium to patients with metastatic melanoma, J. Clin, Oncol. 20: 142-152; Meir et al., 2001, Phase 1 trial of a live, attenuated Salmonella typhimurium (VNP20009) administered by direct Intra-tumoral (IT) injection, Proc Am Soc Clin Oncol 20: abstr 1043); Nemunaitis et al., 2003, Pilot trial of genetically modified, attenuated Salmonella expressing the E. coli cytosine deaminase gene in refractory cancer patients, Cancer Gene Therapy 10: 737-744) is that no significant antitumor activity has been observed, even in patients where the bacteria was documented to target the tumor. One method of increasing the ability of the bacteria to kill tumor cells is to engineer the bacteria to express conventional bacterial toxins (e.g., WO 2009/126189, WO 03/014380, WO/2005/018332, WO/2008/073148, US 2003/0059400 U.S. Pat. Nos. 7,452,531, 7,354,592, 6,962,696, 6,923,972, 6,863,894, 6,685,935, 6,475,482, 6,447,784, 6,190,657 and 6,080,849, 8,241,623, 8,524,220 8,771,669, 8,524,220).

Use of secreted proteins in live bacterial vectors has been demonstrated by several authors. Holland et al. (U.S. Pat. No. 5,143,830) have illustrated the use of fusions with the C-terminal portion of the hemolysin A (hlyA) gene, a member of the type I secretion system. When co-expressed in the presence of the hemolysin protein secretion channel (hlyBD) and a functional TolC, heterologous fusions are readily secreted from the bacteria. The type I secretion system that has been utilized most widely, and although it is currently considered the best system available, is thought to have limitations for delivery by attenuated bacteria (Hahn and Specht, 2003, FEMS Immunology and Medical Microbiology, 37: 87-98). Those limitations include the amount of protein secreted and the ability of the protein fused to it to interfere with secretion. Improvements of the type I secretion system have been demonstrated by Sugamata and Shiba (2005 Applied and Environmental Microbiology 71: 656-662), using a modified hlyB, and by Gupta and Lee (2008 Biotechnology and Bioengineering, 101: 967-974), by addition of rare codons to the hlyA gene. Fusion to the gene ClyA (Galen et al., 2004, Infection and Immunity, 72: 7096-7106 and Type III secretion proteins have also been used. Surface display has been used to export proteins outside of the bacteria. For example, fusion of the Lpp protein amino acids 1-9 with the transmembrane region B3-B7 of OmpA has been used for surface display (Samuelson et al., 2002, Display of proteins on bacteria, J. Biotechnology 96: 129-154). The autotransporter surface display has been described by Berthet et al., WO/2002/070645.

Other heterologous protein secretion systems utilizing the autotransporter family can be modulated to result in either surface display or complete release into the medium (see Henderson et al., 2004, Type V secretion pathway: the autotransporter story, Microbiology and Molecular Biology Reviews 68: 692-744; Jose, 2006 Applied Microbiol. Biotechnol. 69: 607-614; Jose J, Zangen D (2005) Autodisplay of the protease inhibitor aprotinin in Escherichia coli. Biochem Biophys Res Commun 333:1218-1226 and Rutherford and Mourez 2006 Microbial Cell Factories 5: 22). For example, Veiga et al. (2003 Journal of Bacteriology 185: 5585-5590 and Klauser et al., 1990 EMBO Journal 9: 1991-1999), demonstrated hybrid proteins containing the b-autotransporter domain of the immunoglobulin A (IgA) protease of Nisseria gonorrhea.

Fusions to flagellar proteins have been demonstrated. The peptide, usually of 15 to 36 amino acids in length, is inserted into the central, hypervariable region of the FliC gene such as that from Salmonella muenchen (Verma et al. 1995 Vaccine 13: 235-24; Wu et al., 1989 Proc. Natl. Acad. Sci. USA 86: 4726-4730; Cuadro et al., 2004 Infect. Immun. 72: 2810-2816; Newton et al., 1995, Res. Microbiol. 146: 203-216, each of which is expressly incorporated by reference in its entirety). Multihybrid FliC insertions of up to 302 amino acids have also been prepared (Tanskanen et al. 2000, Appl. Env. Microbiol. 66: 4152-4156). Trimerization of antigens and functional proteins can be achieved using the T4 fibritin foldon trimerization sequence (Wei et al. 2008 J. Virology 82: 6200-6208) and VASP tetramerization domains (Kühnel et al., 2004 PNAS 101: 17027-17032). The multimerization domains are used to create, bi-specific, tri-specific, and quatra-specific targeting agents, whereby each individual agent is expressed with a multimerization tag, each of which may have the same or separate targeting peptide, such that following expression, surface display, secretion and/or release, they form multimers with multiple targeting domains. Other secretion systems include C-terminal fusions to the protein YebF (Zhang et al., 2006, Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli, Nat Biotechnol 24: 100-104), which is commercially available as a kit (pAES40; AthenaES, Baltimore, Md.). Fusions to OmsY and other proteins are also capable of secreting proteins into the medium (Zian et al., 2008, Proteome-Based Identification of Fusion Partner for High-Level Extracellular Production of Recombinant Proteins in Escherichia coli, Biotechnol Bioegineer 101: 587-601). Other secretions systems usable according to the present invention include that of Kotzsch et al. 2011 (A secretory system for bacterial production of high-profile protein targets, Protein Science 20: 597-609) using OmpA, OmpF and OsmY, or those described by Yoon et al., 2010 (Secretory production of recombinant proteins in Escherichia coli, Recent patents on Biotechnology 4: 23-29. See, US2006-7094579, WO2009021548, EP1402036, US2006-7070989, US2008/0193974, US2006-7052867, US2003-6605697, U.S. Pat. No. 5,470,719, US2007/0287171, US2009/0011995, US2008/0076157, US2006-7112434, US2005-6919198, US2002-6455279, US2007-7291325, US2008-7410788, US2000-6083715, EP1270730, US2004-6673569, US2001-6309861, U.S. Pat. No. 5,989,868, US2006-7056732, US2005-6852512, US2005-6861403, EP1407052, WO2008089132, U.S. Pat. No. 5,824,502, EP1068339B1, US2008/0166757, US2001-6329172, US2003-6596509, US2003-6642027, WO2006017929, US2003-6596510, US2008/0280346, US2007-7202059, US2008/0280346, US2007-7202059, US2009-7491528, US2008/0206814, US2008/0166764, US2008/0182295, US2008/0254511, US2008/0206818, US2006-7105327, US2004/0005695, U.S. Pat. No. 5,508,192, EP866132, U.S. Pat. Nos. 6,921,659, 6,828,121, US2008/0064062, EP786009, US2006/0270043, and U.S. Pat. No. 7,202,059.

Compositions described in accordance with various embodiments herein include, without limitation, Salmonella enterica serovar Typhimurium (“S. typhimurium”), Salmonella montevideo, Salmonella enterica serovar Typhi (“S. typhi”), Salmonella enterica serovar Paratyphi A, Paratyphi B (“S. paratyphi B”), Salmonella enterica serovar Paratyphi C (“S. paratyphi C”), Salmonella enterica serovar Hadar (“S. hadar”), Salmonella enterica serovar Enteriditis (“S. enteriditis”), Salmonella enterica serovar Kentucky (“S. kentucky”), Salmonella enterica serovar Infantis (“S. infantis”), Salmonella enterica serovar Pullorum (“S. pullorum”), Salmonella enterica serovar Gallinarum (“S. gallinarum”), Salmonella enterica serovar Muenchen (“S. muenchen”), Salmonella enterica serovar Anatum (“S. anatum”), Salmonella enterica serovar Dublin (“S. dublin”), Salmonella enterica serovar Derby (“S. derby”), Salmonella enterica serovar Choleraesuis var. kunzendorf (“S. cholerae kunzendorf”), and Salmonella enterica serovar minnesota (S. minnesota).

By way of example, live bacteria in accordance with aspects of the invention include known strains of S. enterica serovar Typhimurium (S. typhimurium) and S. enterica serovar Typhi (S. typhi) which are further modified as provided by various embodiments of the invention. Such Strains include Ty21a, CMV906, CMV908, CMV906-htr, CMV908-htr, Ty800, aroA-/serC-, holavax, M01ZH09, VNP20009. These strains contain defined mutations within specific serotypes of bacteria. The technology also includes the use of these same (or different) mutational combinations contained within alternate serotypes or strains in order to avoid immune reactions which may occur in subsequent administrations. For example, S. Typhimurium, S. montevideo, and S. typhi which have non-overlapping O-antigen presentation (e.g., S. typhimurium is O-1, 4, 5, 12 and S. typhi is Vi, S. montevideo is O-6, 7) may be used. Thus, for example, S. typhimurium is a suitable serotype for a first administration and another serotype such as S. typhi or S. montevideo are used for a second administration and third administration. Likewise, the flagellar antigens are also selected for non-overlapping antigenicity between different administrations. The flagellar antigen may be H1 or H2 or no flagellar antigen, which, when combined with the three different O-antigen serotypes, provides three completely different antigenic profiles.

Novel strains are also encompassed that are, for example, attenuated in virulence by mutations in a variety of metabolic and structural genes. The invention therefore may provide a live composition for treating cancer comprising a live attenuated bacterium that is a serovar of Salmonella enterica comprising an attenuating mutation in a genetic locus of the chromosome of said bacterium that attenuates virulence of said bacterium and wherein said attenuating mutation is the Suwwan deletion (Murray et al., 2004. Hot spot for a large deletion in the 18-19 Cs region confers a multiple phenotype in Salmonella enterica serovar Typhimurium strain ATCC 14028. Journal of Bacteriology 186: 8516-8523 (2004)) or combinations with other known attenuating mutations. Other attenuating mutation useful in the Salmonella bacterial strains described herein may be in a genetic locus selected from the group consisting of phoP, phoQ, edt, cya, crp, poxA, rpoS, htrA, nuoG, pmi, pabA, pts, damA, met, cys, pur, purA, purB, purl, purF, zwf, aroA, aroB, aroC, aroD, serC, gua, cadA, rfc, rjb, rfa, ompR, msbB, leucine and arginine, pfkAB, crr, glk, ptsG, ptsHI, manXYZ and combinations thereof. Strains of Salmonella deleted in stn are particularly preferred.

Attenuated gram-positive bacteria are also available as delivery vectors. For example, Staphylococcus epidermidis, group B Streptococcus including S. agalaciae, and Listeria species including L. monocytogenes may be employed. It is known to those skilled in the art that variations in molecular biology techniques such as use of gram-positive origins of replication, gram-positive signal sequences and gram-positive promoters and filamentous phage (e.g., phage B5; Chopin et al., 2002 J. Bacteriol. 184: 2030-2033, described further below) may be employed and substituted as needed. Other bacterial strains may also be encompassed, including non-pathogenic bacteria of the gut skin (such as Staphylococcus epidermidis, Proprionibacteria) and other body locations known as the human microbiome (Grice et al., Topographical and temporal diversity of the human skin microbiome, Science 324: 1190-1192; A framework for human microbiome research; The Human Microbiome Project Consortium, 14 Jun. 2012 Nature 486, 215-221; Spor et al., 2011, Unravelling the effects of the environment and host genotype on the gut microbiome, Nature Reviews Microbiology 9: 279-290) such as E. coli strains, Bacteriodies, Bifidobacterium and Bacillus, attenuated pathogenic strains of E. coli including enteropathogenic and uropathogenic isolates, Enterococcus sp. and Serratia sp. as well as attenuated Neisseria sp., Shigella sp., Staphylococcus sp., Staphylococcus carnosis, Yersinia sp., Streptococcus sp. and Listeria sp. including L. monocytogenes. Bacteria of low pathogenic potential to humans and other mammals or birds or wild animals, pets and livestock, such as insect pathogenic Xenorhabdus sp., Photorhabdus sp. and human wound Photorhabdus (Xenorhabdus) are also encompassed. Probiotic strains of bacteria are also encompassed, including Lactobacillus sp. (e.g., Lactobacillus acidophilus, Lactobacillus salivarius) Lactococcus sp., (e.g., Lactococcus lactis, Lactococcus casei) Leuconostoc sp., Pediococcus sp., Streptococcus sp. (e.g., S. salivariu, S. thermophilus), Bacillus sp., Bifidobacterium sp., Bacteroides sp., and Escherichia coli such as the 1917 Nissel strain.

It is known to those skilled in the art that minor variations in molecular biology techniques such as use of gram-positive origins of replication, gram-positive signal sequences gram-positive promoters (e.g., Lactococcus expression, Mohamadzadeh et al., PNAS Mar. 17, 2009 vol. 106 no. 11 4331-4336) may be used and substituted as needed. The bacteria may be further modified to be internalized into the host cell (Guimaraes et al., 2006, Use of Native Lactococci as Vehicles for Delivery of DNA into Mammalian Epithelial Cells, Appl Environ Microbiol. 2006 November; 72(11): 7091-7097; Innocentin et al., 2009, Lactococcus lactis Expressing either Staphylococcus aureus Fibronectin-Binding Protein A or Listeria monocytogenes Internalin A Can Efficiently Internalize and Deliver DNA in Human Epithelial Cells Appl Environ Microbiol. 2009 July; 75(14): 4870-4878).

Recently developed approaches to delivery of therapeutic molecules (U.S. Pat. Nos. 8,241,623; 8,524,220; 8,771,669; and 8,524,220) have coupled a protease sensitive therapeutic molecule with co-expression of protease inhibitors, expressly incorporated by reference herein.

The autotransporter surface display has been described by Berthet et al., WO/2002/070645, expressly incorporated by reference herein. Other heterologous protein secretion systems utilizing the autotransporter family can be modulated to result in either surface display or complete release into the medium (see Henderson et al., 2004, Type V secretion pathway: the autotransporter story, Microbiology and Molecular Biology Reviews 68: 692-744; Jose, 2006 Applied Microbiol. Biotechnol. 69: 607-614; Jose J, Zangen D (2005) Autodisplay of the protease inhibitor aprotinin in Escherichia coli. Biochem Biophys Res Commun 333:1218-1226 and Rutherford and Mourez 2006 Microbial Cell Factories 5: 22). For example, Veiga et al. (2003 Journal of Bacteriology 185: 5585-5590 and Klauser et al., 1990 EMBO Journal 9: 1991-1999) demonstrated hybrid proteins containing the β-autotransporter domain of the immunoglobulin A (IgA) protease of Nisseria gonorrhea. Fusions to flagellar proteins have been demonstrated. The peptide, usually of 15 to 36 amino acids in length, is inserted into the central, hypervariable region of the FliC gene such as that from Salmonella muenchen (Verma et al. 1995 Vaccine 13: 235-24; Wu et al., 1989 Proc. Natl. Acad. Sci. USA 86: 4726-4730; Cuadro et al., 2004 Infect. Immun. 72: 2810-2816; Newton et al., 1995, Res. Microbiol. 146: 203-216, expressly incorporated by reference in their entirety herein). Multihybrid FliC insertions of up to 302 amino acids have also been prepared (Tanskanen et al. 2000, Appl. Env. Microbiol. 66: 4152-4156, expressly incorporated by reference in its entirety herein).

Trimerization of antigens can be achieved using the T4 fibritin foldon trimerization sequence (Wei et al. 2008 J. Virology 82: 6200-6208) and VASP tetramerization domains (Kühnel et al., 2004 PNAS 101: 17027-17032), expressly incorporated by reference in their entirety herein. The multimerization domains are used to create, bi-specific, tri-specific, and quatra-specific targeting agents, whereby each individual agent is expressed with a multimerization tag, each of which may have the same or separate targeting peptide, such that following expression, surface display, secretion and/or release, they form multimers with multiple targeting domains. A fusion with the Pseudomonas ice nucleation protein (INP) wherein the N- and C-terminus of INP with an internal deletion consisting of the first 308 amino acids is followed by the mature sequence of the protein to be displayed (Jung et al., 1998, Surface display of Zymomonas mobilis levansucrase by using ice-nucleation protein of Pseudomonas syringae, Nature Biotechnology 16: 576-580; Kim et al., 2000, Bacterial surface display of an enzyme library for selective screening of improved cellulase variants, Applied and Environmental Microbiology 66: 788-793; Part:BBa_K811003 from www.iGEM.org; WO2005005630).

Salmonella are also encompassed that are, for example, attenuated in virulence by mutations in a variety of metabolic and structural genes. The technology therefore may provide a live composition for treating cancer comprising a live attenuated bacterium that is a serovar of Salmonella enterica comprising an attenuating mutation in a genetic locus of the chromosome of said bacterium that attenuates virulence of said bacterium and wherein said attenuating mutation is a combinations of other known attenuating mutations. The strain may also contain a mutation known as “Suwwan”, which is an approximately 100 kB deletion between two IS200 elements. The strain may also carry a defective thioredoxin gene (trxA-; which may be used in combination with a TrxA fusion), a defective glutathione oxidoreductase (gor-) and optionally, overexpress a protein disulfide bond isomerase (DsbA). The strain may also be engineered to express invasion and/or escape genes tlyA, tlyC patI and pld from Rickettsia, whereby the bacteria exhibit enhanced invasion and/or escape from the phagolysosome (Witworth et al., 2005, Infect. Immun. 73:6668-6673), thereby enhancing the activity of the effector genes described below. The strain may also be engineered to be deleted in an avirulence (anti-virulence) gene, such as zirTS, grvA and/or pcgL, or express the E. coli lac repressor, which is also an avirulence gene in order to compensate for over-attenuation. The strain may also express SlyA, a known transcriptional activator. In a preferred embodiment, the Salmonella strains are msbB mutants (msbB⁻). In a more preferred embodiment, the strains are msbB- and Suwwan. In a more preferred embodiment the strains are msbB⁻, Suwwan and zwf⁻. Zwf has recently been shown to provide resistance to CO2, acidic pH and osmolarity (Karsten et al., 2009, BMC Microbiology August 18; 9:170). Use of the msbB zwf genetic combination is also particularly preferred for use in combination with administered carbogen (an oxygen carbon dioxide mixture that may enhance delivery of therapeutic agents to a tumor). In a more preferred embodiment, the strains are msbB⁻, Suwwan, zwf⁻ and trxA⁻. In a most preferred embodiment, the strains are msbB⁻, Suwwan, zwf⁻, trxA⁻ and gor.

The technology also provides, according to one embodiment, a process for preparing genetically stable therapeutic bacterial strains comprising genetically engineering the therapeutic genes of interest into a bacterially codon optimized expression sequence within a bacterial plasmid expression vector, endogenous virulence (VIR) plasmid (of Salmonella sp.), or chromosomal localization expression vector for any of the deleted genes or IS200 genes, defective phage or intergenic regions within the strain and further containing engineered restriction endonuclease sites such that the bacterially codon optimized expression gene contains subcomponents which are easily and rapidly exchangeable, and the bacterial strains so produced. The present technology provides, for example, and without limitation, live bacterial compositions that are genetically engineered to express one or more protease inhibitors combined with antigens.

According to various embodiments, the technology provides pharmaceutical compositions comprising pharmaceutically acceptable carriers and one or more bacterial mutants. The technology also provides pharmaceutical compositions comprising pharmaceutically acceptable carriers and one or more bacterial mutants comprising nucleotide sequences encoding one or more peptides. Preferably, the bacterial mutants are attenuated by introducing one or more mutations in one or more genes in the lipopolysaccharide (LPS) biosynthetic pathway (for gram-negative bacteria), and optionally one or more mutations to auxotrophy for one or more nutrients or metabolites.

In one embodiment, a pharmaceutical composition comprises a pharmaceutically acceptable carrier and one or more bacterial mutants, wherein said attenuated bacterial mutants are facultative anaerobes or facultative aerobes. In another embodiment, a pharmaceutical composition comprises a pharmaceutically acceptable carrier and one or more attenuated bacterial mutants, wherein said attenuated bacterial mutants are facultative anaerobes or facultative aerobes. In one embodiment, a pharmaceutical composition comprises a pharmaceutically acceptable carrier and one or more bacterial mutants, wherein said attenuated bacterial mutants are facultative anaerobes or facultative aerobes. In another embodiment, a pharmaceutical composition comprises a pharmaceutically acceptable carrier and one or more attenuated bacterial mutants, wherein said attenuated bacterial mutants are facultative anaerobes or facultative aerobes.

A pharmaceutically effective dosage form may comprise between about 10⁵ to 10¹² live bacteria, within a lyophilized medium for oral administration. In some embodiments, about 10⁹ live bacteria are administered.

Pharmaceutically acceptable formulations may be provided for delivery by other various routes e.g., by intramuscular injection, subcutaneous delivery, by intranasal delivery (e.g., WO 00/47222, U.S. Pat. No. 6,635,246), intradermal delivery (e.g., WO02/074336, WO02/067983, WO02/087494, WO02/0832149 WO04/016281, each of which is expressly incorporated herein by reference it its entirety) by transdermal delivery, by transcutaneous delivery, by topical routes, etc. Injection may involve a needle (including a microneedle), or may be needle-free. See, e.g., U.S. Pat. Nos. 7,452,531, 7,354,592, 6,962,696, 6,923,972, 6,863,894, 6,685,935, 6,475,482, 6,447,784, 6,190,657, 6,080,849 and US Pub. 2003/0059400, each of which is expressly incorporated herein by reference.

Bacterial vector vaccines are known, and similar techniques may be used for the present bacteria as for bacterial vaccine vectors (U.S. Pat. No. 6,500,419, Curtiss, In: New Generation Vaccines: The Molecular Approach, Ed., Marcel Dekker, Inc., New York, N.Y., pages 161-188 and 269-288 (1989); and Mims et al, In: Medical Microbiology, Eds., Mosby-Year Book Europe Ltd., London (1993)). These known vaccines can enter the host, either orally, intranasally or parenterally. Once gaining access to the host, the bacterial vector vaccines express an engineered prokaryotic expression cassette contained therein that encodes a foreign antigen(s). Foreign antigens can be any protein (or part of a protein) or combination thereof from a bacterial, viral, or parasitic pathogen that has vaccine properties (New Generation Vaccines: The Molecular Approach, supra; Vaccines and Immunotherapy, supra; Hilleman, Dev. Biol. Stand., 82:3-20 (1994); Formal et al, Infect. Immun. 34:746-751 (1981); Gonzalez et al, J. Infect. Dis., 169:927-931 (1994); Stevenson et al, FEMS Lett., 28:317-320 (1985); Aggarwal et al, J. Exp. Med., 172:1083-1090 (1990); Hone et al, Microbial. Path., 5:407-418 (1988); Flynn et al, Mol. Microbiol., 4:2111-2118 (1990); Walker et al, Infect. Immun., 60:4260-4268 (1992); Cardenas et al, Vacc., 11:126-135 (1993); Curtiss et al, Dev. Biol. Stand., 82:23-33 (1994); Simonet et al, Infect. Immun., 62:863-867 (1994); Charbit et al, Vacc., 11:1221-1228 (1993); Turner et al, Infect. Immun., 61:5374-5380 (1993); Schodel et al, Infect. Immun., 62:1669-1676 (1994); Schodel et al, J. Immunol., 145:4317-4321 (1990); Stabel et al, Infect. Immun., 59:2941-2947 (1991); Brown, J. Infect. Dis., 155:86-92 (1987); Doggett et al, Infect. Immun., 61:1859-1866 (1993); Brett et al, Immunol., 80:306-312 (1993); Yang et al, J. Immunol., 145:2281-2285 (1990); Gao et al, Infect. Immun., 60:3780-3789 (1992); and Chatfield et al, Bio/Technology, 10:888-892 (1992)). Delivery of the foreign antigen to the host tissue using bacterial vector vaccines results in host immune responses against the foreign antigen, which provide protection against the pathogen from which the foreign antigen originates (Mims, The Pathogenesis of Infectious Disease, Academic Press, London (1987); and New Generation Vaccines: The Molecular Approach, supra). See also: Formal et al, Infect. Immun., 34:746-751 (1981); Wick et al, Infect. Immun., 62:4542-4548 (1994)); Hone et al, Vaccine, 9:810-816 (1991); Tacket et al, Infect. Immun., 60:536-541 (1992); Hone et al, J. Clin. Invest., 90:412-420 (1992); Chatfield et al, Vaccine, 10:8-11 (1992); Tacket et al, Vaccine, 10:443-446 (1992); van Damme et al, Gastroenterol., 103:520-531 (1992) (Yersinia pestis), Noriega et al, Infect. Immun., 62:5168-5172 (1994) (Shigella spp.), Levine et al, In: Vibrio cholerae, Molecular to Global Perspectives, Wachsmuth et al, Eds, ASM Press, Washington, D.C., pages 395-414 (1994) (Vibrio cholerae), Lagranderie et al, Vaccine, 11:1283-1290 (1993); Flynn, Cell. Molec. Biol., 40 (Supp1.1):31-36 (1994) (Mycobacterium strain BCG), Schafer et al, J. Immunol., 149:53-59 (1992)(Listeria monocytogenes), each of which is expressly incorporated herein by reference.

The bacteria are generally administered along with a pharmaceutically acceptable carrier and/or diluent. The particular pharmaceutically acceptable carrier and/or diluent employed is not critical to the present invention unless otherwise specific herein (or in a respective incorporated referenced relevant to the issue). Examples of diluents include a phosphate buffered saline, buffer for buffering against gastric acid in the stomach, such as citrate buffer (pH 7.0) containing sucrose, bicarbonate buffer (pH 7.0) alone (Levine et al, J. Clin. Invest., 79:888-902 (1987); and Black et al J. Infect. Dis., 155:1260-1265 (1987), expressly incorporated herein by reference), or bicarbonate buffer (pH 7.0) containing ascorbic acid, lactose, and optionally aspartame (Levine et al, Lancet, 11:467-470 (1988), expressly incorporated herein by reference). Examples of carriers include proteins, e.g., as found in skim milk, sugars, e.g., sucrose, or polyvinylpyrrolidone. Typically, these carriers would be used at a concentration of about 0.1-30% (w/v) but preferably at a range of 1-10% (w/v).

Set forth below are other pharmaceutically acceptable carriers or diluents which may be used for delivery specific routes. Any such carrier or diluent can be used for administration of the bacteria of the invention, so long as the bacteria are still capable of invading a target cell. In vitro or in vivo tests for invasiveness can be performed to determine appropriate diluents and carriers. The compositions of the invention can be formulated for a variety of types of administration, including systemic and topical or localized administration. Lyophilized forms are also included, so long as the bacteria are invasive upon contact with a target cell or upon administration to the subject. Techniques and formulations generally may be found in Remington's Pharmaceutical Sciences, Meade Publishing Co., Easton, Pa., expressly incorporated herein by reference in its entirety. For systemic administration, injection is preferred, including intramuscular, intravenous, intraperitoneal, and subcutaneous. For injection, the composition, e.g., bacteria, of the invention can be formulated in liquid solutions, preferably in physiologically compatible buffers such as Hank's solution or Ringer's solution.

For oral administration, the pharmaceutical compositions may take the form of, for example, tablets or capsules prepared by conventional means with pharmaceutically acceptable excipients such as binding agents (e.g., pregelatinized maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose); fillers (e.g., lactose, microcrystalline cellulose or calcium hydrogen phosphate); lubricants (e.g., magnesium stearate, talc or silica); disintegrants (e.g., potato starch or sodium starch glycolate); or wetting agents (e.g., sodium lauryl sulfate). The tablets may be coated by methods well known in the art. Liquid preparations for oral administration may take the form of, for example, solutions, syrups or suspensions, or they may be presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations may be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents (e.g., sorbitol syrup, cellulose derivatives or hydrogenated edible fats); emulsifying agents (e.g., lecithin or acacia); non-aqueous vehicles (e.g., almond oil, oily esters, ethyl alcohol or fractionated vegetable oils); and preservatives. The preparations may also contain buffer salts, flavoring, coloring and sweetening agents as appropriate.

Preparations for oral administration may be suitably formulated to give controlled release or enteric release. For buccal administration the compositions may take the form of tablets or lozenges formulated in conventional manner.

For administration by inhalation, the pharmaceutical compositions for use according to the present invention are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebulizer, with the use of a suitable propellant, e.g., a hydrofluorocarbon (HFC), carbon dioxide or other suitable gas. In the case of a pressurized aerosol the dosage unit may be determined by providing a valve to deliver a metered amount. Capsules and cartridges of e.g., gelatin for use in an inhaler or insufflator may be formulated containing a powder mix of the composition, e.g., bacteria, and a suitable powder base such as lactose or starch.

The pharmaceutical compositions may be formulated for parenteral administration by injection, e.g., by bolus injection or continuous infusion. Formulations for injection may be presented in unit dosage form, e.g., in ampoules or in multi-dose containers, with an added preservative. The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents. Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g., sterile pyrogen-free water, before use.

See also U.S. Pat. No. 6,962,696, expressly incorporated herein by reference in its entirety.

The present invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and an attenuated tumor-targeted bacteria comprising one or more nucleic acid molecules encoding one or more primary effector molecules operably linked to one or more appropriate promoters. The present invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and an attenuated tumor-targeted bacteria comprising one or more nucleic acid molecules encoding one or more primary effector molecules and one or more secondary effector molecules operably linked to one or more appropriate promoters.

The present invention provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a bacterium.

In a specific embodiment, the term “pharmaceutically acceptable” means approved by a regulatory agency of the Federal or a state government or listed in the U.S. Pharmacopeia or other generally recognized pharmacopeia for use in animals, and more particularly in humans. The term “carrier” refers to a diluent, adjuvant, excipient, or vehicle with which the therapeutic is administered. Such pharmaceutical carriers can be sterile liquids, such as water and oils, including those of petroleum, animal, vegetable or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil, olive oil, and the like. Saline is a preferred carrier when the pharmaceutical composition is administered intravenously. Saline solutions and aqueous dextrose and glycerol solutions can also be employed as liquid carriers, particularly for injectable solutions. Suitable pharmaceutical excipients include starch, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, sodium stearate, glycerol monostearate, talc, sodium chloride, dried skim milk, glycerol, propylene, glycol, water, and the like. The composition, if desired, can also contain minor amounts of wetting or emulsifying agents, or pH buffering agents. These compositions can take the form of solutions, suspensions, emulsion, tablets, pills, capsules, powders, sustained-release formulations and the like. Oral formulation can include standard carriers such as pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharine, cellulose, magnesium carbonate, etc. Examples of suitable pharmaceutical carriers are described in “Remington's Pharmaceutical Sciences” by E. W. Martin. Such compositions will contain a therapeutically effective amount of the therapeutic attenuated tumor-targeted bacteria, in purified form, together with a suitable amount of carrier so as to provide the form for proper administration to the patient. The formulation should suit the mode of administration.

In a preferred embodiment, the composition is formulated in accordance with routine procedures as a pharmaceutical composition adapted for intravenous administration to human beings. Typically, compositions for intravenous administration are solutions in sterile isotonic aqueous buffer. Where necessary, the composition may also include a suspending agent and a local anesthetic such as lignocaine to ease pain at the site of the injection. Generally, the ingredients are supplied either separately or mixed together in unit dosage form, for example, as a dry lyophilized powder or water free concentrate in a hermetically sealed container such as an ampoule or sachette indicating the quantity of active agent. Where the composition is to be administered by infusion, it can be dispensed with an infusion bottle containing sterile pharmaceutical grade water or saline. Where the composition is administered by injection, an ampoule of sterile water for injection or saline can be provided so that the ingredients may be mixed prior to administration.

The amount of the pharmaceutical composition of the invention which will be effective in the vaccination of a subject can be determined by standard clinical techniques. In addition, in vitro assays may optionally be employed to help identify optimal dosage ranges. The precise dose to be employed in the formulation will also depend on the route of administration, and should be decided according to the judgment of the practitioner and each patient's circumstances. However, suitable dosage ranges are generally from about 1.0 cfu/kg to about 1×10¹⁰ cfu/kg; optionally from about 1.0 cfu/kg to about 1×10⁸ cfu/kg; optionally from about 1×10² cfu/kg to about 1×10⁸ cfu/kg; optionally from about 1 10⁴ cfu/kg to about 1×10⁸ cfu/kg; and optionally from about 1×10⁴ cfu/kg to about 1×10¹⁰ cfu/kg (cfu=colony forming unit). Effective doses may be extrapolated from dose-response curves derived from in vitro or animal model test systems.

Various delivery systems are known and can be used to administer a pharmaceutical composition of the present invention. Methods of introduction include but are not limited to intradermal, intramuscular, intraperitoneal, intravenous, subcutaneous, intranasal, and oral routes. The compositions may be administered by any convenient route, for example by infusion or bolus injection, by absorption through epithelial or mucocutaneous linings (e.g., oral mucosa, rectal and intestinal-mucosa, etc.) and may be administered together with other biologically active agents. Administration can be systemic or local. Pulmonary administration can also be employed, e.g., by use of an inhaler or nebulizer, and formulation with an aerosolizing agent.

The invention also provides a pharmaceutical pack or kit comprising one or more containers filled with one or more of the ingredients of the pharmaceutical compositions of the invention. Optionally associated with such container(s) can be a notice in the form prescribed by governmental agency regulating the manufacture, use or sale of pharmaceuticals or biological products, which notice reflects approval by the agency of manufacture, use or sale for human administration.

The compositions and methods described herein can be administered to a subject in need of treatment, e.g., in need of treatment for inflammation or cancer. In some embodiments, the methods described herein comprise administering an effective amount of compositions described herein, e.g., engineered microbial cells to a subject in order to alleviate a symptom. As used herein, “alleviating a symptom” is ameliorating any condition or symptom associated with a given condition. As compared with an equivalent untreated control, such reduction is by at least 5%, 10%, 20%, 40%, 50%, 60%, 80%, 90%, 95%, 99% or more as measured by any standard technique. A variety of means for administering the compositions described herein to subjects are known to those of skill in the art. Such methods can include, but are not limited to oral, subcutaneous, transdermal, airway (aerosol), cutaneous, topical, or injection administration. Administration can be local or systemic.

The term “effective amount” as used herein refers to the amount of engineered microbial cells needed to alleviate at least one or more symptom of the disease or disorder, and relates to a sufficient amount of pharmacological composition to provide the desired effect. The term “therapeutically effective amount” therefore refers to an amount of engineered microbial cells that is sufficient to effect a particular effect when administered to a typical subject. An effective amount as used herein, in various contexts, would also include an amount sufficient to delay the development of a symptom of the disease, alter the course of a symptom disease (for example but not limited to, slowing the progression of a symptom of the disease), or reverse a symptom of the disease. Thus, it is not generally practicable to specify an exact “effective amount”. However, for any given case, an appropriate “effective amount” can be determined by one of ordinary skill in the art using only routine experimentation.

Effective amounts, toxicity, and therapeutic efficacy can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the ED₅₀ (the dose therapeutically effective in 50% of the population). The dosage can vary depending upon the dosage form employed and the route of administration utilized. The dose ratio between toxic and therapeutic effects is the therapeutic index and can be expressed as the ratio ED₅₀. Compositions and methods that exhibit large therapeutic indices are preferred. A therapeutically effective dose can be estimated initially from cell culture assays. Also, a dose can be formulated in animal models to achieve a circulating plasma concentration range that includes the IC₅₀ (i.e., the concentration of an engineered microbial cell which achieves a half-maximal inhibition of symptoms) as determined in cell culture, or in an appropriate animal model. Levels in plasma can be measured, for example, by high performance liquid chromatography. The effects of any particular dosage can be monitored by a suitable bioassay, e.g., assay for inflammation, among others. The dosage can be determined by a physician and adjusted, as necessary, to suit observed effects of the treatment.

In some embodiments, the technology described herein relates to a pharmaceutical composition comprising an engineered microbial cell as described herein, and optionally a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers and diluents include saline, aqueous buffer solutions, solvents and/or dispersion media. The use of such carriers and diluents is well known in the art. Some non-limiting examples of materials which can serve as pharmaceutically-acceptable carriers include: (1) sugars, such as lactose, glucose and sucrose; (2) starches, such as corn starch and potato starch; (3) cellulose, and its derivatives, such as sodium carboxymethyl cellulose, methylcellulose, ethyl cellulose, microcrystalline cellulose and cellulose acetate; (4) powdered tragacanth; (5) malt; (6) gelatin; (7) lubricating agents, such as magnesium stearate, sodium lauryl sulfate and talc; (8) excipients, such as cocoa butter and suppository waxes; (9) glycols, such as propylene glycol; (10) polyols, such as glycerin, sorbitol, mannitol and polyethylene glycol (PEG); (11) esters, such as ethyl oleate and ethyl laurate; (12) agar; (13) buffering agents, such as magnesium hydroxide and aluminum hydroxide; (14) alginic acid; (15) pyrogen-free water; (16) isotonic saline; (17) Ringer's solution; (18) pH buffered solutions; (19) polyesters, polycarbonates and/or polyanhydrides; (20) bulking agents, such as polypeptides and amino acids (21) serum component, such as serum albumin, HDL and LDL; and (22) other non-toxic compatible substances employed in pharmaceutical formulations. Wetting agents, coloring agents, release agents, coating agents, sweetening agents, flavoring agents, perfuming agents, preservative and antioxidants can also be present in the formulation. The terms such as “excipient”, “carrier”, “pharmaceutically acceptable carrier” or the like are used interchangeably herein.

Pharmaceutical compositions comprising an engineered microbial cell can be formulated to be suitable for oral administration, for example as discrete dosage forms, such as, but not limited to, tablets (including without limitation scored or coated tablets), pills, caplets, capsules, chewable tablets, powder packets, cachets, troches, wafers, aerosol sprays, or liquids, such as but not limited to, syrups, elixirs, solutions or suspensions in an aqueous liquid. Such compositions contain a predetermined amount of the pharmaceutically acceptable salt of the disclosed compounds, and may be prepared by methods of pharmacy well known to those skilled in the art. See generally, Remington: The Science and Practice of Pharmacy, 21st Ed., Lippincott, Williams, and Wilkins, Philadelphia Pa. (2005).

In certain embodiments, an effective dose of a composition comprising engineered microbial cells as described herein can be administered to a patient once. In certain embodiments, an effective dose of a composition comprising engineered microbial cells can be administered to a patient repeatedly. In some embodiments, the dose can be a daily administration, for example oral administration, of, e.g., a capsule comprising bacterial cells as described herein. In some embodiments, the dose can be, e.g., an injection or gavage of bacterial cells. In some embodiments, the dose can be administered systemically, e.g., by intravenous injection. In some embodiments, a dose can comprise from 10⁶ to 10¹² cells. In some embodiments, a dose can comprise from about 10⁸ to 10¹⁰ cells. A composition comprising engineered microbial cells can be administered over a period of time, such as over a 5 minute, 10 minute, 15 minute, 20 minute, or 25 minute period. The administration can be repeated, for example, on a regular basis, such as every few days, once a week, or biweekly (i.e., every two weeks) for one month, two months, three months, four months or longer.

In some embodiments, after an initial treatment regimen, the treatments can be administered on a less frequent basis. For example, after treatment biweekly for three months, treatment can be repeated once per month, for six months or a year or longer.

The efficacy of engineered microbial cells in, e.g., the raising of an appropriate immune response to a specified disease, e.g., COVID-19, can be determined by the skilled clinician. However, a treatment is considered “effective treatment,” as the term is used herein, clinically useful partial or complete immunity is achieved. Efficacy can be assessed, for example, by measuring a marker, indicator, population statistic, or any other measurable parameter appropriate.

For convenience, the meaning of some terms and phrases used in the specification, examples, and appended claims, are provided below. Unless stated otherwise, or implicit from context, the following terms and phrases include the meanings provided below. The definitions are provided to aid in describing particular embodiments, and are not intended to limit the claimed invention, because the scope of the invention is limited only by the claims. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. If there is an apparent discrepancy between the usage of a term in the art and its definition provided herein, the definition provided within the specification shall prevail.

For convenience, certain terms employed herein, in the specification, examples and appended claims are collected here.

The terms “decrease”, “reduced”, “reduction”, or “inhibit” are all used herein to mean a decrease by a statistically significant amount. In some embodiments, the terms “reduced”, “reduction”, “decrease”, or “inhibit” can mean a decrease by at least 10% as compared to a reference level, for example a decrease by at least about 20%, or at least about 30%, or at least about 40%, or at least about 50%, or at least about 60%, or at least about 70%, or at least about 80%, or at least about 90% or more or any decrease of at least 10% as compared to a reference level. In some embodiments, the terms can represent a 100% decrease, i.e., a non-detectable level as compared to a reference level. In the context of a marker or symptom, a “decrease” is a statistically significant decrease in such level. The decrease can be, for example, at least 10%, at least 20%, at least 30%, at least 40% or more, and is preferably down to a level accepted as within the range of normal for an individual without such disorder. In some instances, the symptom can be essentially eliminated which means that the symptom is reduced, i.e., the individual is in at least temporary remission.

The terms “increased”, “increase”, “enhance”, or “activate” are all used herein to mean an increase by a statically significant amount. In some embodiments, the terms “increased”, “increase”, “enhance”, or “activate” can mean an increase of at least 10% as compared to a reference level, for example an increase of at least about 20%, or at least about 30%, or at least about 40%, or at least about 50%, or at least about 60%, or at least about 70%, or at least about 80%, or at least about 90% or up to and including a 100% increase or any increase between 10-100% as compared to a reference level, or at least about a 2-fold, or at least about a 3-fold, or at least about a 4-fold, or at least about a 5-fold or at least about a 10-fold increase, or any increase between 2-fold and 10-fold or greater as compared to a reference level. In the context of a marker or symptom, a “increase” is a statistically significant increase in such level.

As used herein, a “subject” means a human or non-human animal. Usually, the non-human animal is a vertebrate such as a primate, rodent, domestic animal or game animal. Primates include chimpanzees, cynomolgous monkeys, spider monkeys, and macaques, e.g., Rhesus. Rodents include mice, rats, woodchucks, ferrets, rabbits and hamsters. Animals also include armadillos, hedgehogs, and camels, to name a few. Domestic and game animals include cows, horses, pigs, deer, bison, buffalo, feline species, e.g., domestic cat, canine species, e.g., dog, fox, wolf, avian species, e.g., chicken, emu, ostrich, and fish, e.g., trout, catfish and salmon. In some embodiments, the subject is a mammal, e.g., a primate, e.g., a human. The terms, “individual,” “patient” and “subject” are used interchangeably herein.

Preferably, the subject is a mammal. The mammal can be a human, non-human primate, mouse, rat, dog, cat, horse, cow, or pig, but is not limited to these examples. Mammals other than humans can be advantageously used as subjects that represent animal models of a given condition. A subject can be male or female.

A subject can be one who has been previously diagnosed with or identified as suffering from or having a condition in need of treatment, and optionally, have already undergone treatment. Alternatively, a subject can also be one who has not been previously diagnosed as having a condition. For example, a subject can be one who exhibits one or more risk factors or a subject who does not exhibit risk factors.

A “subject in need” of treatment for a particular condition can be a subject having that condition, diagnosed as having that condition, or at risk of developing that condition.

As used herein, the terms “protein” and “polypeptide” are used interchangeably herein to designate a series of amino acid residues, connected to each other by peptide bonds between the alpha-amino and carboxy groups of adjacent residues. The terms “protein”, and “polypeptide” refer to a polymer of amino acids, including modified amino acids (e.g., phosphorylated, glycated, glycosylated, etc.) and amino acid analogs, regardless of its size or function. “Protein” and “polypeptide” are often used in reference to relatively large polypeptides, whereas the term “peptide” is often used in reference to small polypeptides, but usage of these terms in the art overlaps. The terms “protein” and “polypeptide” are used interchangeably herein when referring to a gene product and fragments thereof. Thus, exemplary polypeptides or proteins include gene products, naturally occurring proteins, homologs, orthologs, paralogs, fragments and other equivalents, variants, fragments, and analogs of the foregoing.

As used herein, the term “nucleic acid” or “nucleic acid sequence” refers to any molecule, preferably a polymeric molecule, incorporating units of ribonucleic acid, deoxyribonucleic acid or an analog thereof. The nucleic acid can be either single-stranded or double-stranded. A single-stranded nucleic acid can be one strand nucleic acid of a denatured double-stranded DNA. Alternatively, it can be a single-stranded nucleic acid not derived from any double-stranded DNA. In one aspect, the nucleic acid can be DNA. In another aspect, the nucleic acid can be RNA. Suitable nucleic acid molecules are DNA, including genomic DNA or cDNA. Other suitable nucleic acid molecules are RNA, including mRNA.

The term “expression” refers to the cellular processes involved in producing RNA and proteins and as appropriate, secreting proteins, including where applicable, but not limited to, for example, transcription, transcript processing, translation and protein folding, modification and processing. “Expression products” include RNA transcribed from a gene, and polypeptides obtained by translation of mRNA transcribed from a gene. The term “gene” means the nucleic acid sequence which is transcribed (DNA) to RNA in vitro or in vivo when operatively linked to appropriate regulatory sequences. A gene may or may not include regions preceding and following the coding region, e.g., 5′ untranslated (5′UTR) or “leader” sequences and 3′ UTR or “trailer” sequences.

The term “operatively linked” includes having an appropriate start signal (e.g., ATG) in front of the polynucleotide sequence to be expressed, and maintaining the correct reading frame to permit expression of the polynucleotide sequence under the control of the expression control sequence, and, optionally, production of the desired polypeptide encoded by the polynucleotide sequence. In some examples, transcription of a nucleic acid is under the control of a promoter sequence (or other transcriptional regulatory sequence) which controls the expression of the nucleic acid in a cell-type in which expression is intended. It will also be understood that the nucleic acid can be under the control of transcriptional regulatory sequences which are the same or which are different from those sequences which control transcription of the naturally-occurring form of a protein.

The term “isolated” or “partially purified” as used herein refers, in the case of a nucleic acid or polypeptide, to a nucleic acid or polypeptide separated from at least one other component (e.g., nucleic acid or polypeptide) that is present with the nucleic acid or polypeptide as found in its natural source and/or that would be present with the nucleic acid or polypeptide when expressed by a cell, or secreted in the case of secreted polypeptides. A chemically synthesized nucleic acid or polypeptide or one synthesized using in vitro transcription/translation is considered “isolated.”

As used herein, the terms “treat,” “treatment,” “treating,” or “amelioration” refer to therapeutic treatments, wherein the object is to reverse, alleviate, ameliorate, inhibit, slow down or stop the progression or severity of a condition associated with a disease or disorder, e.g., cancer or inflammation. The term “treating” includes reducing or alleviating at least one adverse effect or symptom of a condition, disease or disorder. Treatment is generally “effective” if one or more symptoms or clinical markers are reduced. Alternatively, treatment is “effective” if the progression of a disease is reduced or halted. That is, “treatment” includes not just the improvement of symptoms or markers, but also a cessation of, or at least slowing of, progress or worsening of symptoms compared to what would be expected in the absence of treatment. Beneficial or desired clinical results include, but are not limited to, alleviation of one or more symptom(s), diminishment of extent of disease, stabilized (i.e., not worsening) state of disease, delay or slowing of disease progression, amelioration or palliation of the disease state, remission (whether partial or total), and/or decreased mortality, whether detectable or undetectable. The term “treatment” of a disease also includes providing relief from the symptoms or side-effects of the disease (including palliative treatment).

As used herein, the term “pharmaceutical composition” refers to the active agent in combination with a pharmaceutically acceptable carrier e.g., a carrier commonly used in the pharmaceutical industry. The phrase “pharmaceutically acceptable” is employed herein to refer to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.

As used herein, the term “administering,” refers to the placement of a compound as disclosed herein into a subject by a method or route which results in at least partial delivery of the agent at a desired site. Pharmaceutical compositions comprising the compounds disclosed herein can be administered by any appropriate route which results in an effective treatment in the subject.

The term “statistically significant” or “significantly” refers to statistical significance and generally means a two-standard deviation (2SD) or greater difference.

Other than in the operating examples, or where otherwise indicated, all numbers expressing quantities of ingredients or reaction conditions used herein should be understood as modified in all instances by the term “about.” The term “about” when used in connection with percentages can mean±1%.

As used herein the term “comprising” or “comprises” is used in reference to compositions, methods, and respective component(s) thereof, that are essential to the method or composition, yet open to the inclusion of unspecified elements, whether essential or not.

The term “consisting of” refers to compositions, methods, and respective components thereof as described herein, which are exclusive of any element not recited in that description of the embodiment.

As used herein the term “consisting essentially of” refers to those elements required for a given embodiment. The term permits the presence of elements that do not materially affect the basic and novel or functional characteristic(s) of that embodiment.

The singular terms “a,” “an,” and “the” include plural referents unless context clearly indicates otherwise. Similarly, the word “or” is intended to include “and” unless the context clearly indicates otherwise. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of this disclosure, suitable methods and materials are described below. The abbreviation, “e.g.” is derived from the Latin exempli gratia, and is used herein to indicate a non-limiting example. Thus, the abbreviation “e.g.” is synonymous with the term “for example.”

Definitions of common terms in cell biology and molecular biology can be found in “The Merck Manual of Diagnosis and Therapy”, 19th Edition, published by Merck Research Laboratories, 2006 (ISBN 0-911910-19-0); Robert S. Porter et al. (eds.), The Encyclopedia of Molecular Biology, published by Blackwell Science Ltd., 1994 (ISBN 0-632-02182-9); Benjamin Lewin, Genes X, published by Jones & Bartlett Publishing, 2009 (ISBN-10: 0763766321); Kendrew et al. (eds.), Molecular Biology and Biotechnology: a Comprehensive Desk Reference, published by VCH Publishers, Inc., 1995 (ISBN 1-56081-569-8) and Current Protocols in Protein Sciences 2009, Wiley Intersciences, Coligan et al., eds.

Unless otherwise stated, the present invention was performed using standard procedures, as described, for example in Sambrook et al., Molecular Cloning: A Laboratory Manual (3 ed.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., USA (2001); Davis et al., Basic Methods in Molecular Biology, Elsevier Science Publishing, Inc., New York, USA (1995); Current Protocols in Protein Science (CPPS) (John E. Coligan, et. al., ed., John Wiley and Sons, Inc.), Current Protocols in Cell Biology (CPCB) (Juan S. Bonifacino et. al. ed., John Wiley and Sons, Inc.), and Culture of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, Publisher: Wiley-Liss; 5th edition (2005), Animal Cell Culture Methods (Methods in Cell Biology, Vol. 57, Jennie P. Mather and David Barnes editors, Academic Press, 1st edition, 1998) which are all incorporated by reference herein in their entireties.

Other terms are defined herein within the description of the various aspects of the invention.

All patents and other publications; including literature references, issued patents, published patent applications, and co-pending patent applications; cited throughout this application are expressly incorporated herein by reference for all purposes, including, but not limited to, describing and disclosing, for example, the methodologies described in such publications that might be used in connection with the technology described herein.

SUMMARY OF THE INVENTION

The recent worldwide outbreak of SARS-CoV-2 has created an urgent need for protective vaccines. Major targets for vaccines against coronaviruses have been previously identified as the capsid, nucleoprotein, and the Spike or S glycoprotein. Among these, the receptor binding domain (RBD) of the spike protein of SARS-CoV plays a particularly important role because it is necessary for viral binding to the protein ACE2 linked to entry into the host. With the goal of eliciting neutralizing antibodies, and because the bat RBD differs from the SARS-CoV-2, and that the entire protein contains immunodominant epitopes that do not include the SARS-CoV-2 RBD, analysis was focused on predicted MHC Class II responses to the RBD itself. Based on these analyses, an attenuated bacterial delivery vector (VNP20009) that has previously been safely administered in human clinical studies, was selected to express secreted, YebF fusions to different portions of the RBD.

The present invention provides a vaccine directed toward the prevention of COVID19 caused by SARS-CoV-2. A DNA construct is provided in a live bacterium, such as E. coli Nissel 1917 or attenuated strains of Salmonella such as Salmonella typhimurium VNP20009. The DNA construct may uniquely represent the receptor binding domain (RBD) of SARS-Cov-2. The live bacterium carrying the DNA construct may be administered orally to a patient or an animal (such as a bat species that could be a host for SARS-CoV-2) in order to induce protective immunity.

A preferred embodiment of the design of the DNA construct is shown in FIG. 1.

Protective immunity through prior exposure is a natural mechanism that limits pandemic spread of emerging pathogens. However, as periodically seen with influenza, immune escape through antigenic drift, or more dramatically, through antigenic shift, allows reemergence of new broadly infective variants over time. These variants are shaped in part by preexisting immunity that limits the more immunologically similar variants and creates an evolutionary opportunity for immunologically dissimilar variants that retain transmissibility.

Severe acute respiratory syndrome (SARS) caused by SARS-CoV coronavirus emerged in 2003 as a new, high mortality respiratory infection. SARS-CoV is believed to have a zoonotic origin, as similar coronaviruses have been found in a number of animal species including bats, which have the highest sequence similarities (Lau et al., 2005; Li et al., 2005).

Antibodies against SARS-CoV S protein have been shown to be neutralizing (Kapada et al., 2005). Previous studies have shown that the spike protein of SARS-CoV induces long-term protective immunity (Du et al., 2007 Vaccine. 2007 Apr. 12; 25(15):2832-8). In that study, the receptor domain was fused with the human IgG1 Fc region. Others have included rhabdovirus-based vectors carrying the SARS-CoV spike protein (Faber et al., 2005), and baculovirus expressed proteins Zhou et al., 2006). Current approaches have recently been summarized (Le et al., 2020). As of that review, there were 78 different projects, most still in the exploratory stage. The mechanisms of immunization and/or antigen delivery include live attenuated viruses, inactivated viruses, non-replicating viral vectors, replicating viral vectors, recombinant proteins, peptide-based antigens, virus-like particles, DNA and RNA. Of the five projects that have moved to the clinic, three include vaccinating against the spike S glycoprotein: an mRNA to the entire 1273 amino acid sequence (Moderna; NCT04283461), an adenovirus type 5 vector (CanSino Biologics; NCT04313127), and a plasmid DNA vector (Inovio Pharmaceuticals; NCT04336410). In that review, the mechanisms of immunization and/or antigen delivery being investigated included live attenuated viruses, inactivated viruses, non-replicating viral vectors, replicating viral vectors, recombinant proteins, peptide-based antigens, virus-like particles, DNA and RNA, while bacterial vectors were not included.

Live attenuated bacterial vectors have been used to generate heterologous vaccines. In a recent study, the Salmonella vector VNP20009 (YS1646) that had been developed as a cancer therapeutic (Low et al., 2004) was employed as a delivery vector for an antigen from Clostridium difficile (Winter et al., 2019). Clostridium difficile is the causative agent for pseudomembranous enterocolitis, an often-fatal disease, yet protection of 82% was achieved with oral immunization and up to 100% using multimodal immunization. Live attenuated bacterial vaccines have many potential advantages, including rapid molecular engineering and rapid production due to cell-free replication competence, and low cost of production.

Alternative approaches to vaccine design continue to be explored. In a recent publication by Grifoni et al. (2020), sequence homology between SARS-CoV and SARS-CoV-2 was used to predict vaccine candidates. Although this approach may generate an immune response, there is only limited evidence that there is any cross protection of SARS-CoV and SARS-CoV-2, and thus the immune response may not be effective. Furthermore, Ou et al. (2020) have now shown that there the antibodies SARS-CoV show limited cross-neutralization of SARS-CoV-2. In addition, there is growing concern that infection by and SARS-CoV-2 does not protect against and SARS-CoV-2 reinfection. It may be that the dominant antibodies produced help with immune clearance, but don't prevent infection because they don't block internalization.

SARS-CoV and SARS-CoV-2 share amino acid sequence homology and therefore a common ancestor, although the closest homology is with bats (Wu 2020, doi.org/10.1101/2020.03.04.975995). It therefore seems most likely that SARS-CoV-2 emerged from bats rather than from SARS-CoV. While there are many sequence differences between SARS-CoV-2 and the bat coronavirus, there are a particularly striking number of differences within the receptor binding domain. These differences are likely to contribute to lack of cross-immunity between SARS-CoV and SARS-CoV-2 (Ou et al., 2020), and are also likely to contribute to the cross over to humans and the highly infectious nature of SARS-CoV-2 toward humans, which begins with its interaction with human ACE2 (hACE2). If true, the RBD alteration responsible for host specificity and antigen escape are linked. Three mutant types (V367F, W436R, and D364Y) in the RBD showed higher binding affinity to human ACE2 (Ou et al. Emergence of RBD mutations in circulating SARS-CoV-2 strains enhancing the structural stability and human ACE2 receptor affinity of the spike protein, bioRxiv preprint doi: doi.org/10.1101/2020.03.15.991844).

The present approach is guided by the following presumptions:

That and SARS-CoV-2 is derived from the bat coronavirus.

-   -   That the mutations in the RBD result in increased human         specificity.     -   That the mutations in the RBD result in immune escape.

That antibodies against the SARS-CoV-2 RBD would have the greatest microneutralization potential.

That the SARS-CoV-2 spike protein contains immunodominant epitopes that may limit the immune response to the RBD.

-   -   That immunization with the RBD only removes the immunodominant         epitopes and will result in an RBD-directed response.     -   Thus, immunization against the SARS-CoV-2 RBD would be more         effective than the spike protein alone.

That the SARS-CoV-2 RBD can be expressed and secreted by an attenuated bacterial vector as a fusion protein such as with YebF or HlyA.

-   -   That the fusion protein is capable of eliciting a neutralizing         MHC Class II response.

That an attenuated bacterial vector expressing the SARS-CoV-2 RBD, such as VNP20009 can be safe and effective for administration to humans.

It is therefore an object to provide a live genetically engineered bacterium, comprising a genetically engineered construct comprising a nucleic acid sequence encoding at least one portion of a SARS-CoV-2 antigen, the live genetically engineered bacterium being adapted for administration to a human or animal and colonization of at least one tissue under non-lethal conditions.

It is a further object to provide a genetically engineered bacterium selected from the group consisting of E. coli and Salmonella, comprising: a first genetically engineered construct comprising a nucleic acid sequence encoding at least one portion of a SARS-CoV-2 spike protein receptor binding domain, having an associated promoter; and a second genetically engineered construct comprising a nucleic acid sequence encoding an adjuvant peptide. The at least one portion of a SARS-CoV-2 spike protein receptor binding domain and the adjuvant peptide may be together expressed as a fusion peptide.

It is a still further object to provide a method of vaccinating a human against SARS-CoV-2, comprising: administering the live genetically engineered bacterium according to claim 1 orally, intranasally, or rectally to the human or animal; allowing the live genetically engineered bacterium to colonize a tissue of the human or animal; and clearing the live genetically engineered bacterium from the human or animal, wherein said administration, colonization, and clearance are non-lethal to the human or animal. The at least one portion of a SARS-CoV-2 antigen may comprise the SARS-Cov-2 spike protein, and the nucleic acid sequence encoding the SARS-CoV-2 spike protein may have an associated promoter.

The at least one portion of a SARS-CoV-2 antigen may comprise the SARS-Cov-2 spike protein.

The nucleic acid sequence encoding the SARS-CoV-2 spike protein may have an associated promoter.

The nucleic acid sequence may further encode a secretion signal.

The live genetically engineered bacterium may be E. coli, e.g., E. coli Nissel 1917, or Salmonella, e.g., Salmonella typhimurium or VNP20009/YS1646.

The at least one portion of the SARS-CoV-2 spike gene portion may be an antigenic subportion of the receptor binding domain.

The at least one portion of the SARS-CoV-2 spike gene portion may be fused in-frame with an adjuvant peptide encoding sequence.

The adjuvant peptide may be a dimer of at least a portion of p28.

The adjuvant peptide may be selected from the group consisting of flagellin, a subportion of flagellin that binds to a toll-like receptor, a combination of p28 and flagellin toll-like receptor binding region, a dimer of C3d p28, and a dimer of C3d p28 with an internal flagellin peptide.

The live genetically engineered bacterium may express a gut colonization factor.

The gut colonization factor may be selected from the group consisting of colicin A, E1, E2, E3, E4, E5, E6, E7, E8, E9, DF13, K, N, U, B, D, Ia, and M.

The nucleic acid sequence may further encode at least one of an angiotensin converting enzyme 2 binding peptide and a portion of angiotensin binding protein 2.

The nucleic acid sequence may encode a fusion protein further comprising a portion selected from the group consisting of YebF, ice nucleation protein, autodisplay proteins and HlyA.

The fusion protein may be a truncated portion containing a one or more disulfide bonds or lacking potential for such bonds.

The live genetically engineered bacterium may co-express a colicin immunity peptide and a colicin lysis peptide.

The nucleic acid sequence may be integrated with a bacterial chromosome.

The nucleic acid sequence may encode a secretion signal.

The nucleic acid sequence may encode a protease inhibitor, e.g., a serpin or furin inhibitor.

The colonization factor may enhance immunization. The colonization factor may be a colicin co-expressed with its immunity and lysis peptides. The colonization factor may be ColE3.

The bacteria may produce a fusion peptide comprising the antigen and an adjuvant peptide.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows a plasmid vector for expression of SARS-CoV-2 Receptor Binding Domain in E. coli and Salmonella vaccine delivery vectors.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

DNA sequence analysis of RBD and its mutations Recently, a study of the global sequence variation within SARS-CoV-2 has been made available in an interactive format by NextStrain. Their analysis shows eight major strains circulating across the globe, with strong geographic preferences, suggesting the specific mutations they carry represent those of the progenitors for most of the strains in those regions.

The receptor binding domain of SARS-CoV-2, with 4 added amino acids (SEQ ID NO. 001 CGPK) predicted to be antigenic using the online analysis tool EMBOSS. The additional cysteine may provide for disulfide bonding.

SEQ ID NO. 002 NITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKC YGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDD FTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGS TPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCG PK

A graphical representation of the modified portion of the circular plasmid pTrc99a (Genbank U13872.1). The lacI (repressor) has been partially deleted. Various portions of the receptor binding domain RBD of SARS-CoV-2 (Wuhan-Hu-1 NC_045512.2), e.g., 488-525, preceded by a flexible linker, are expressed under control of the ptrc promoter with an RBS as an in-frame fusion with YebF (e.g., Quintero et al., 2018, Co-expression of a chimeric protease inhibitor secreted by a tumor-targeted Salmonella protects therapeutic proteins from proteolytic degradation, J Microbiol Biotechnol 2018 Dec. 28; 28(12):2079-2094. doi: 10.4014/jmb.1807.08036), followed by a stop codon. Receptor binding domain may include the cysteines for possible disulfide bonding. Optionally, and adjuvant peptide such as C3d-P28208-235 (Groot et al., 2015, C3d adjuvant effects are mediated through the activation of C3d-specific autoreactive T cells, Immunol Cell Biol 93(2): 189-197. doi:10.1038/icb.2014.89), or flagellin peptides (e.g., those of domains aD1a, aD1b, aD1C, Song et al., 2017, A conserved TLR5 binding and activation hot spot on flagellin, Scientific Reports 7, Article number: 40878 (2017)), may be inserted singly or at both sites to create repeats. A colonization enhancing factor, colicin E3 (E3) and its immunity factor (E3 immune) from Genbank KM287568 are optionally co-expressed.

The receptor binding domain of SARS-CoV-2, residues 488-525 (hereinafter “SARS-Cov-2 RBD 488-525”) is:

SEQ ID NO. 003 CYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVC

Flexible linker (SEQ ID NO. 004 GGGGS) with SARS-Cov-2 RBD 488-525:

SEQ ID NO. 005 GGGGScyfplqsygfqptngvgyqpyrvvvlsfellhapatvc

FIG. 1 shows plasmid vector for expression of SARS-CoV-2 Receptor Binding Domain in E. coli and Salmonella vaccine delivery vectors. A graphical representation of the modified portion of the circular plasmid pTrc99a (Genbank U13872.1). The lad (repressor) has been partially deleted. Various portions of the receptor binding domain RBD of SARS-CoV-2 (Wuhan-Hu-1 NC_045512.2), e.g., 488-525, preceded by a flexible linker, are expressed under control of the ptrc promoter with an RBS as an in-frame fusion with YebF (e.g., Quintero et al., 2018, Co-expression of a chimeric protease inhibitor secreted by a tumor-targeted Salmonella protects therapeutic proteins from proteolytic degradation, J Microbiol Biotechnol 2018 Dec. 28; 28(12):2079-2094. doi: 10.4014/jmb.1807.08036), followed by a stop codon. Receptor binding domain may include the cysteines for possible disulfide bonding. Optionally, and adjuvant peptide such as C3d-P28208-235. The flexible linker with RBD 488-528 may be, for example,

Flexible linker (SEQ ID NO. 004 GGGGS), followed by SARS-Cov-2 RBD 488-525 SEQ ID NO. 003, followed by GPK:

SEQ ID NO. 006 GGGGScyfplqsygfqptngvgyqpyrvvvlsfellhapatvcGPK

C3d-P28208-235:

SEQ ID NO. 007 KFLTTAKDKNRWEDPGKQLYNVEATSYA

Groot et al., 2015, C3d adjuvant effects are mediated through the activation of C3d-specific autoreactive T cells, Immunol Cell Biol 93(2): 189-197. doi:10.1038/icb.2014.89), or flagellin peptides (e.g., those of domains aD1a, aD1b, aD1C, Song et al., 2017, A conserved TLR5 binding and activation hot spot on flagellin, Scientific Reports 7, Article number: 40878 (2017)), may be inserted singly or at both sites to create repeats. A colonization enhancing factor, colicin E3 (E3) and its immunity factor (E3 immune) from Genbank KM287568 are optionally co-expressed.

Example 1

A yebF fusion with 41 amino acids of the RBD, expressed by an attenuated Salmonella VNP20009.

An expression plasmid, pTrc99a, with a YebF, containing an in-frame fusion with a portion of the RBD consisting of 41 amino acids, from a cysteine to a cysteine with added GPK and an added artificial lysine followed by a stop codon.

SEQ ID NO. 008 atggctaaaaaaagaggggcgtttttagggctgttgttggtttc tgcctgcgcatcagttttcgctgccaataatgaaaccagcaagt cggtcactttcccaaagtgtgaagatctggatgctgccggaatt gccgcgagcgtaaaacgtgattatcaacaaaatcgcgtggcgcg ttgggcagatgatcaaaaaattgtcggtcaggccgatcccgtgg cttgggtcagtttgcaggacattcagggtaaagatgataaatgg tcagtaccgctaaccgtgcgtggtaaaagtgccgatattcatta ccaggtcagcgtggactgcaaagcgggaatggcggaatatcagc ggcgt ctcgagGGTactagtGGCGGTGGTGGCAGTtgcTATTTT CCACTGCAGTOTTATGGCTTTCAGCCGACTAACGGTGTGGGTTA CCAACCGTACCGTGTGGTTGTACTGTCTTTCGAGCTGCTGCATG CCCCGGCAACCGTATGCGgCCCGAAGAAATCTtga

An example of a complete pTrc99a plasmid, with the YebF 41aa of RBD, and the ColE3 colicin, immunity and lysis protein:

SEQ ID NO. 009 GTTTGACAGCTTATCATCGACTGCACGGTGCACCAATGCTTCTGGCGTCAGGCAGC CATCGGAAGCTGTGGTATGGCTGTGCAGGTCGTAAATCACTGCATAATTCGTGTCGCT CAAGGCGCACTCCCGTTCTGGATAATGTTTTTTGCGCCGACATCATAACGGTTCTGGC AAATATTCTGAAATGAGCTGTTGACAATTAATCATCCGGCTCGTATAATGTGTGGAAT TGTGAGCGGATAACAATTTCACACAGGAAACAGA CC atggctaaaaaaagaggggcgt ttttagggctgttgttggtttctgcctgcgcatcagttttcgctgccaataatgaaac cagcaagtcggtcactttcccaaagtgtgaagatctggatgctgccggaattgccgcg agcgtaaaacgtgattatcaacaaaatcgcgtggcgcgttgggcagatgatcaaaaaa ttgtcggtcaggccgatcccgtggcttgggtcagtttgcaggacattcagggtaaaga tgataaatggtcagtaccgctaaccgtgcgtggtaaaagtgccgatattcattaccag gtcagcgtggactgcaaagcgggaatggcggaatatcagcggcgtctcgagGGTacta gtGGCGGTGGTGGCAGTtgcTATTTTCCACTGCAGTCTTATGGCTTTCAGCCGACTAA CGGTGTGGGTTACCAACCGTACCGTGTGGTTGTACTGTCTTTCGAGCTGCTGCATGCC CCGGCAACCGTATGCGgCCCGAAGAAATCTtgaTCTAGAGTCGACCTGCAGGCATGCA AGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGA ACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCA CCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGT CTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGA AAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGAC AAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCA GGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATG GCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATG TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGA GTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCT TCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTT TTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGC GGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGA CAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTT ACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACG ACGAGCGTGACACCACGATGCCTACAGCAATGGCAACAACGTTGCGCAAACTATTAAC TGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATA AATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGG TAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAG ACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAG GATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTT TCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTT TTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGT TTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGA GCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGA ACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGC CAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAG GCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGA CCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGA AGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACG AGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACC TCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAA CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG TTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAG CTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGC GGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGc atatgcccgctctgcgttttctaagtgttatccctcctgatttctaaaaaattttcca cctgaacTTGACagaaaaaacgatgacgagtactttttgatctgtacataaacccagt ggttttatgtacagtattaatcgtgtaatcaattgttttaaCgCttaaaagagggaat ttttatgagcggtggcgatggacgcggccataacacgggcgcgcatagcacaagtggt aacattaatggtggcccgaccgggcttggtgtaggtggtggtgcttctgatggctccg gatggagttcggaaaataacccgtggggtggtggttccggtagcggcattcactgggg tggtggttccggtcatggtaatggcggggggaatggtaattccggtggtggttcggga acaggcggtaatctgtcagcagtagctgcgccagtggcatttggttttccggcacttt ccactccaggagctggcggtctggcggtcagtatttcagcgggagcattatcggcagc tattgctgatattatggctgccctgaaaggaccgtttaaatttggtctttggggggtg gctttatatggtgtattgccatcacaaatagcgaaagatgaccccaatatgatgtcaa agattgtgacgtcattacccgcagatgatattactgaatcacctgtcagttcattacc tctcgataaggcaacagtaaacgtaaatgttcgtgttgttgatgatgtaaaagacgag cgacagaatatttcggttgtttcaggtgttccgatgagtgttccggtggttgatgcaa aacctaccgaacgtccgggtgtttttacggcatcaattccaggtgcacctgttctgaa tatttcagttaataacagtacgccagcagtacagacattaagcccaggtgttacaaat aatactgataaggatgttcgcccggcaggatttactcagggtggtaataccagggatg cagttattcgattcccgaaggacagcggtcataatgccgtatatgtttcagtgagtga tgttcttagccctgaccaggtaaaacaacgtcaagatgaagaaaatcgccgtcagcag gaatgggatgctacgcatccggttgaagcggctgagcgaaattatgaacgcgcgcgtg cagagctgaatcaggcaaatgaagatgttgccagaaatcaggagcgacaggctaaagc tgttcaggtttataattcgcgtaaaagcgaacttgatgcagcgaataaaactcttgct gatgcaatagctgaaataaaacaatttaatcgatttgcccatgacccaatggctggcg gtcacagaatgtggcaaatggccgggcttaaagcccagcgggcgcagacggatgtaaa taataagcaggctgcatttgatgctgctgcaaaagagaagtcagatgctgatgctgca ttgagttctgctatggaaagcaggaagaagaaagaagataagaaaaggagtgctgaaa ataatttaaacgatgaaaagaataagcccagaaaaggttttaaagattacgggcatga ttatcatccagctccgaaaactgagaatattaaagggcttggtgatcttaagcctggg ataccaaaaacaccaaagcagaatggtggtggaaaacgcaagcgctggactggagata aagggcgtaagatttatgagtgggattctcagcatggtgagcttgaggggtatcgtgc cagtgatggtcagcatcttggctcatttgaccctaaaacaggcaatcagttgaaaggt ccagatccgaaacgaaatatcaagaaatatctttgagaggaagttatgggacttaaat tggatttaacttggtttgataaaagtacagaagattttaagggtgaggagtattcaaa agattttggagatgacggttcagttatggaaagtctaggtgtgccttttaaggataat gttaataacggttgctttgatgttatagctgaatgggtacctttgctacaaccatact ttaatcatcaaattgatatttccgataatgagtattttgtttcgtttgattatcqTGA TGGTGATTGGTGA tcaaatattatcagggatgagttgatatacgggcttctagtgttc atggatgaacgctggagcctccaaatgtagaaatgttatattttttattgagttcttg gttataattgctccgcaatgatttaaataagcattatttaaaacattctcaggagagg tgaaggtggagctaaaaaaaagtattggtgattacactgaaaccgaattcaaaaaatt tattgaagacatcatcaattgtgaaggtgatgaaaaaaaacaggatgataacctcgag tattttataaatgttactgagcatcctagtggttctgatctgatttattacccagaag gtaataatgatggtagccctgaaggtgttattaaagagattaaagaatggcgagccgc taacggtaagtcaggatttaaacagggctgaaatatgaatgccggttgtttatggatg aatggctggcattctttcacaacaaggagtcgttatgaaaaaaataacagggattatt ttattgcttcttgcagtcattattctgtctgcatgtcaggcaaactatatccgggatg ttcagggcgggaccgtatctccgtcatcaacagctgaagtgaccggattagcaacgca gtaacccgaaatcctctttgacaaaaacaaagcgtgtcaggct GCGGCCGC CCATTGC TGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACA CCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATC TGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTC GGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCG ATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAA TGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCT GGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTA GTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCA AACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCA GGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACC ACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGC AGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATG TGAGTTAGCGCGAATTGATCTG

The plasmid may further consist of an adjuvant peptide cloned in-frame into the XhoI and SpeI sites, such as the P28 peptide (Servis and Lambris, 1989. C3 synthetic peptides support growth of human Cr2-positive lymphoblastoid B cells. J Immunol 142: 2207-2212; Wang et al., 2004 (Contribution of C3d-P28 repeats to enhancement of immune responses against HBV-preS2/S induced by gene immunization, World J Gastroenterol 10: 2070-2077), C3d-P28208-235 SEQ ID NO 007,

KFLTTAKDKNRWEDPGKQLYNVEATSYA

or a truncated P28 peptide

SEQ ID NO 010 GKQLYNVEATSYA

de Groot et al.2015 Immunol Cell Biol 93: 189-197. doi:10.1038/icb.2014.89.

An example of an alternative peptide adjuvant is a peptide, artificial sequence, containing P28 dimer, separated by a portion of the Vibrio vulnificus flagellin with flexible linkers (Lei et al., 2018 (Application of built-in adjuvants for epitope-based vaccines. PeerJ 2019 Jan. 14; 6:e6185. doi: 10.7717/peerj.6185; Mizel and Bates, 2010 (Flagellin as an adjuvant: Cellular Mechanisms and Potential, J Immunol. 2010 Nov. 15; 185(10): 5677-5682. doi:10.4049/jimmunol.1002156; Song et al., 2017. A conserved TLR5 binding and activation hot spot on flagellin. Scientific Reports DOI: 10.1038/srep40878).

SEQ ID NO. 011 KFLTTAKDKNRWEDPGKQLYNVEATSYAGGGGSGGGGSGGGGSaqt aegamnettnilqrmrdislqsangsnskservaiqeeitalndel nGGGGSGGGGSGGGGSKFLTTAKDKNRWEDPGKQLYNVEATSYA

Example 2

Colicin E3-CA38 Genbank KM287568 (Morales et al., 2014. Accumulation of single-stranded DNA in Escherichia coli carrying the colicin plasmid pColE3-CA3, Plasmid 77: 7-16).

Colicin E3, E3, E8 immunity

SEQ ID NO. 012 catatgcccgctctgcgttttctaagtgttatccctcctgatttctaaaaaattttccac ctgaacTTGACagaaaaaacgatgacgagtactttttgatctgtacataaacccagtggt tttatgtacagtattaatcgtgtaatcaattgttttaacgcttaaaagagggaatttttat gagcggtggcgatggacgcggccataacacgggcgcgcatagcacaagtggtaacatta atggtggcccgaccgggcttggtgtaggtggtggtgcttctgatggctccggatggagtt cggaaaataacccgtggggtggtggttccggtagcggcattcactggggtggtggttccg gtcatggtaatggcggggggaatggtaattccggtggtggttcgggaacaggcggtaatc tgtcagcagtagctgcgccagtggcatttggttttccggcactttccactccaggagctg gcggtctggcggtcagtatttcagcgggagcattatcggcagctattgctgatattatgg ctgccctgaaaggaccgtttaaatttggtctttggggggtggctttatatggtgtattgc catcacaaatagcgaaagatgaccccaatatgatgtcaaagattgtgacgtcattacccg cagatgatattactgaatcacctgtcagttcattacctctcgataaggcaacagtaaacg taaatgttcgtgttgttgatgatgtaaaagacgagcgacagaatatttcggttgtttcag gtgttccgatgagtgttccggtggttgatgcaaaacctaccgaacgtccgggtgtttttac ggcatcaattccaggtgcacctgttctgaatatttcagttaataacagtacgccagcag tacagacattaagcccaggtgttacaaataatactgataaggatgttcgcccggcaggat ttactcagggtggtaataccagggatgcagttattcgattcccgaaggacagcggtcata atgccgtatatgtttcagtgagtgatgttcttagccctgaccaggtaaaacaacgtcaag atgaagaaaatcgccgtcagcaggaatgggatgctacgcatccggttgaagcggctgagc gaaattatgaacgcgcgcgtgcagagctgaatcaggcaaatgaagatgttgccagaaatc aggagcgacaggctaaagctgttcaggtttataattcgcgtaaaagcgaacttgatgcag cgaataaaactcttgctgatgcaatagctgaaataaaacaatttaatcgatttgcccatg acccaatggctggcggtcacagaatgtggcaaatggccgggcttaaagcccagcgggcgc agacggatgtaaataataagcaggctgcatttgatgctgctgcaaaagagaagtcagatg ctgatgctgcattgagttctgctatggaaagcaggaagaagaaagaagataagaaaagga gtgctgaaaataatttaaacgatgaaaagaataagcccagaaaaggttttaaagattacg ggcatgattatcatccagctccgaaaactgagaatattaaagggcttggtgatcttaagc ctgggataccaaaaacaccaaagcagaatggtggtggaaaacgcaagcgctggactggag ataaagggcgtaagatttatgagtgggattctcagcatggtgagcttgaggggtatcgtg ccagtgatggtcagcatcttggctcatttgaccctaaaacaggcaatcagttgaaaggtc cagatccgaaacgaaatatcaagaaatatctttgagaggaagttATGGGACTTAAATTGG ATTTAACTTGGTTTGATAAAAGTACAGAAGATTTTAAGGGTGAGGAGTATTCAAAAGATT TTGGAGATGACGGTTCAGTTATGGAAAGTCTAGGTGTGCCTTTTAAGGATAATGTTAATA ACGGTTGCTTTGATGTTATAGCTGAATGGGTACCTTTGCTACAACCATACTTTAATCATC AAATTGATATTTCCGATAATGAGTATTTTGTTTCGTTTGATTATCGTGATGGTGATTGGT GAGCGGCCGCCCATTGCTGTGG

Colicin E3, E3, E8 immunity+lysis

SEQ ID NO. 013 cccgctctgcgttttctaagtgttatccctcctgatttctaaaaaattttccacctgaac TTGACagaaaaaacgatgacgagtactttttgatctgtacataaacccagtggttttatg tacagtattaatcgtgtaatcaattgttttaacgcttaaaagagggaatttttatgagcg gtggcgatggacgcggccataacacgggcgcgcatagcacaagtggtaacattaatggtg gcccgaccgggcttggtgtaggtggtggtgcttctgatggctccggatggagttcggaaa ataacccgtggggtggtggttccggtagcggcattcactggggtggtggttccggtcatg gtaatggcggggggaatggtaattccggtggtggttcgggaacaggcggtaatctgtcag cagtagctgcgccagtggcatttggttttccggcactttccactccaggagctggcggtc tggcggtcagtatttcagcgggagcattatcggcagctattgctgatattatggctgccc tgaaaggaccgtttaaatttggtctttggggggtggctttatatggtgtattgccatcac aaatagcgaaagatgaccccaatatgatgtcaaagattgtgacgtcattacccgcagatg atattactgaatcacctgtcagttcattacctctcgataaggcaacagtaaacgtaaatg ttcgtgttgttgatgatgtaaaagacgagcgacagaatatttcggttgtttcaggtgttc cgatgagtgttccggtggttgatgcaaaacctaccgaacgtccgggtgtttttacggcat caattccaggtgcacctgttctgaatatttcagttaataacagtacgccagcagtacaga cattaagcccaggtgttacaaataatactgataaggatgttcgcccggcaggatttactc agggtggtaataccagggatgcagttattcgattcccgaaggacagcggtcataatgccg tatatgtttcagtgagtgatgttcttagccctgaccaggtaaaacaacgtcaagatgaag aaaatcgccgtcagcaggaatgggatgctacgcatccggttgaagcggctgagcgaaatt atgaacgcgcgcgtgcagagctgaatcaggcaaatgaagatgttgccagaaatcaggagc gacaggctaaagctgttcaggtttataattcgcgtaaaagcgaacttgatgcagcgaata aaactcttgctgatgcaatagctgaaataaaacaatttaatcgatttgcccatgacccaa tggctggcggtcacagaatgtggcaaatggccgggcttaaagcccagcgggcgcagacgg atgtaaataataagcaggctgcatttgatgctgctgcaaaagagaagtcagatgctgatg ctgcattgagttctgctatggaaagcaggaagaagaaagaagataagaaaaggagtgctg aaaataatttaaacgatgaaaagaataagcccagaaaaggttttaaagattacgggcatg attatcatccagctccgaaaactgagaatattaaagggcttggtgatcttaagcctggga taccaaaaacaccaaagcagaatggtggtggaaaacgcaagcgctggactggagataaag ggcgtaagatttatgagtgggattctcagcatggtgagcttgaggggtatcgtgccagtg atggtcagcatcttggctcatttgaccctaaaacaggcaatcagttgaaaggtccagatc cgaaacgaaatatcaagaaatatctttgagaggaagttatgggacttaaattggatttaa cttggtttgataaaagtacagaagattttaagggtgaggagtattcaaaagattttggag atgacggttcagttatggaaagtctaggtgtgccttttaaggataatgttaataacggtt gctttgatgttatagctgaatgggtacctttgctacaaccatactttaatcatcaaattg atatttccgataatgagtattttgtttcgtttgatt atcgTGATGGTGATTGGTGA tcaa atattatcagggatgagttgatatacgggcttctagtgttcatggatgaacgctggagcc tccaaatgtagaaatgttatattttttattgagttcttggttataattgctccgcaatga tttaaataagcattatttaaaacattctcaggagaggtgaaggtggagctaaaaaaaagt attggtgattacactgaaaccgaattcaaaaaatttattgaagacatcatcaattgtgaa ggtgatgaaaaaaaacaggatgataacctcgagtattttataaatgttactgagcatcct agtggttctgatctgatttattacccagaaggtaataatgatggtagccctgaaggtgtt attaaagagattaaagaatggcgagccgctaacggtaagtcaggatttaaacagggctga aatatgaatgccggttgtttatggatgaatggctggcattctttcacaacaaggagtcgt tatgaaaaaaataacagggattattttattgcttcttgcagtcattattctgtctgcatg tcaggcaaactatatccgggatgttcagggcgggaccgtatctccgtcatcaacagctga agtgaccggattagcaacgcagtaacccgaaatcctctttgacaa aaacaaagcgtgtca ggct

Wuhan seafood market pneumonia virus isolate Wuhan-Hu-1, complete genome

NCBI Reference Sequence: NC_045512.2

SEQ ID NO. 014 MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLH STQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKS NIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHK NNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKN IDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALH RSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALD PLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFN ATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCF TNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNL DSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYF PLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCV NFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDIT PCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYS TGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARS VASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTS VDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQ VKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGF IKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTI TSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAI GKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDI LSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKM SECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTA PAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCD VVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASV VNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLI AIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT

Wuhan spike protein Receptor Binding Domain (RBD) with 4 additional amino acids

SEQ ID NO. 015 NITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTF KCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYK LPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTE IYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELL HAPATVCGPKP

Wang et al., 2004 (Contribution of C3d-P28 repeats to enhancement of immune responses against HBV-preS2/S induced by gene immunization, World J Gastroenterol 10: 2070-2077. A peptide, artificial sequence, containing P28 dimer, separated by a portion of the Vibrio vulnificus flagellin with flexible linkers, SEQ ID NO. 011.

Lei et al., 2018 (Application of built-in adjuvants for epitope-based vaccines. PeerJ 2019 Jan. 14; 6:e6185. doi: 10.7717/peerj.6185.

Mizel and Bates, 2010 (Flagellin as an adjuvant: Cellular Mechanisms and Potential, J Immunol. 2010 Nov. 15; 185(10): 5677-5682. doi:10.4049/jimmunol.1002156.

Song et al., 2017. A conserved TLR5 binding and activation hot spot on flagellin. Scientific Reports DOI: 10.1038/srep40878

Example 3

A yebF fusion with 38 amino acids of the RBD, expressed by an attenuated Salmonella VNP20009. An expression plasmid, pTrc99a, with a YebF, containing an in-frame fusion with a portion of the RBD consisting of 38 amino acids, from a cysteine to a cysteine.

SEQ ID NO. 016 atggctaaaaaaagaggggcgtttttagggctgttgttggtttctgcc tgcgcatcagttttcgctgccaataatgaaaccagcaagtcggtcact ttcccaaagtgtgaagatctggatgctgccggaattgccgcgagcgta aaacgtgattatcaacaaaatcgcgtggcgcgttgggcagatgatcaa aaaattgtcggtcaggccgatcccgtggcttgggtcagtttgcaggac attcagggtaaagatgataaatggtcagtaccgctaaccgtgcgtggt aaaagtgccgatattcattaccaggtcagcgtggactgcaaagcggga atggcggaatatcagcggcgtctcgagGGTactagtGGCGGTGGTGGC AGTtgcTATTTTCCACTGCAGTCTTATGGCTTTCAGCCGACTAACGGT GTGGGTTACCAACCGTACCGTGTGGTTGTACTGTCTTTCGAGCTGCTG CATGCCCCGGCAACCGTATGCtaatctaga

The plasmid may further consist of an adjuvant peptide cloned in-frame into the XhoI and SpeI sites. The plasmid may further comprise a colicin expression operon, such as that consisting of colicin E3, E3 immunity, E8 immunity, and E3 lysis protein. The complete sequence of a plasmid is containing a YebF with in-frame fusions of a truncated P28 (p13), a 38 amino acid portion of the spike protein RBD, with co-expression of E3, E3 immunity, E8 immunity, and E3 lysis protein.

SEQ ID NO. 017 GTTTGACAGCTTATCATCGACTGCACGGTGCACCAATGCTTCTGGCGTC AGGCAGCCATCGGAAGCTGTGGTATGGCTGTGCAGGTCGTAAATCACTG CATAATTCGTGTCGCTCAAGGCGCACTCCCGTTCTGGATAATGTTTTTT GCGCCGACATCATAACGGTTCTGGCAAATATTCTGAAATGAGCTGTTGA CAATTAATCATCCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACA ATTTCACACAGGAAACAGA CC atggctaaaaaaagaggggcgtttttag ggctgttgttggtttctgcctgcgcatcagttttcgctgccaataatga aaccagcaagtcggtcactttcccaaagtgtgaagatctggatgctgcc ggaattgccgcgagcgtaaaacgtgattatcaacaaaatcgcgtggcgc gttgggcagatgatcaaaaaattgtcggtcaggccgatcccgtggcttg ggtcagtttgcaggacattcagggtaaagatgataaatggtcagtaccg ctaaccgtgcgtggtaaaagtgccgatattcattaccaggtcagcgtgg actgcaaagcgggaatggcggaatatcagcggcgtctcgagGGCGGTGG TGGCAGTGGAAAACAATTATACAATGTGGAAGCAACTTCGTACGCAGGC GGCGGTGGTAGCGGCGGCGGCGGAAGCGGCGGTGGCGGTTCTGGCAAGC AACTCTACAATGTCGAGGCCACTTCATACGGCGGTGGTGGCAGTactag tGGCGGTGGTGGCAGTtgcTATTTTCCACTGCAGTCTTATGGCTTTCAG CCGACTAACGGTGTGGGTTACCAACCGTACCGTGTGGTTGTACTGTCTT TCGAGCTGCTGCATGCCCCGGCAACCGTATGCtaatctagaGTCGACCT GCAGGCATGCAAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGC CTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATT TGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTC AGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCG AGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAA GACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCC CGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATT AAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTC TTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGA GTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTG CCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACA GCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGAC GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACT TGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGAC AGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG GCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTT TTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACC GGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT ACAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTA CTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGT TGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCAC TGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGG GAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT GCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATA TACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGT GAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTT TCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTT GAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTT TTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCC TTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACC GCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGT GGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGG ATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAG CTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTA TGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGG TAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGG AAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTT GAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAA ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTT TGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGT ATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCG AGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTA TTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGcccgctc tgcgttttctaagtgttatccctcctgatttctaaaaaattttccacct gaacTTGACagaaaaaacgatgacgagtactttttgatctgtacataaa cccagtggrntatgtacagtattaatcgtgtaatcaattgttttaacgc ttaaaagagggaatttttatgagcggtggcgatggacgcggccataaca cgggcgcgcatagcacaagtggtaacattaatggtggcccgaccgggct tggtgtaggtggtggtgcttctgatggctccggatggagttcggaaaat aacccgtggggtggtggttccggtagcggcattcactggggtggtggtt ccggtcatggtaatggcggggggaatggtaattccggtggtggttcggg aacaggcggtaatctgtcagcagtagctgcgccagtggcatttggtttt ccggcactttccactccaggagctggcggtctggcggtcagtatttcag cgggagcattatcggcagctattgctgatattatggctgccctgaaagg accgtttaaatttggtctttggggggtggctttatatggtgtattgcca tcacaaatagcgaaagatgaccccaatatgatgtcaaagattgtgacgt cattacccgcagatgatattactgaatcacctgtcagttcattacctct cgataaggcaacagtaaacgtaaatgttcgtgttgttgatgatgtaaaa gacgagcgacagaatatttcggttgtttcaggtgttccgatgagtgttc cggtggttgatgcaaaacctaccgaacgtccgggtgtttttacggcatc aattccaggtgcacctgttctgaatatttcagttaataacagtacgcca gcagtacagacattaagcccaggtgttacaaataatactgataaggatg ttcgcccggcaggatttactcagggtggtaataccagggatgcagttat tcgattcccgaaggacagcggtcataatgccgtatatgtttcagtgagt gatgttcttagccctgaccaggtaaaacaacgtcaagatgaagaaaatc gccgtcagcaggaatgggatgctacgcatccggttgaagcggctgagcg aaattatgaacgcgcgcgtgcagagctgaatcaggcaaatgaagatgtt gccagaaatcaggagcgacaggctaaagctgttcaggtttataattcgc gtaaaagcgaacttgatgcagcgaataaaactcttgctgatgcaatagc tgaaataaaacaatttaatcgatttgcccatgacccaatggctggcggt cacagaatgtggcaaatggccgggcttaaagcccagcgggcgcagacgg atgtaaataataagcaggctgcatttgatgctgctgcaaaagagaagtc agatgctgatgctgcattgagttctgctatggaaagcaggaagaagaaa gaagataagaaaaggagtgctgaaaataatttaaacgatgaaaagaata agcccagaaaaggttttaaagattacgggcatgattatcatccagctcc gaaaactgagaatattaaagggcttggtgatcttaagcctgggatacca aaaacaccaaagcagaatggtggtggaaaacgcaagcgctggactggag ataaagggcgtaagatttatgagtgggattctcagcatggtgagcttga ggggtatcgtgccagtgatggtcagcatcttggctcatttgaccctaaa acaggcaatcagttgaaaggtccagatccgaaacgaaatatcaagaaat atctttgagaggaagttatgggacttaaattggatttaacttggtttga taaaagtacagaagattttaagggtgaggagtattcaaaagattttgga gatgacggttcagttatggaaagtctaggtgtgccttttaaggataatg ttaataacggttgctttgatgttatagctgaatgggtacctttgctaca accatactttaatcatcaaattgatatttccgataatgagtattttgtt tcgtttgattatcgTGATGGTGATTGGTGA tcaaatattatcagggatg agttgatatacgggcttctagtgttcatggatgaacgctggagcctcca aatgtagaaatgttatattttttattgagttcttggttataattgctcc gcaatgatttaaataagcattatttaaaacattctcaggagaggtgaag gtggagctaaaaaaaagtattggtgattacactgaaaccgaattcaaaa aatttattgaagacatcatcaattgtgaaggtgatgaaaaaaaacagga tgataacctcgagtattttataaatgttactgagcatcctagtggttct gatctgatttattacccagaaggtaataatgatggtagccctgaaggtg ttattaaagagattaaagaatggcgagccgctaacggtaagtcaggatt taaacagggctgaaatatgaatgccggttgtttatggatgaatggctgg cattctttcacaacaaggagtcgttatgaaaaaaataacagggattatt ttattgcttcttgcagtcattattctgtctgcatgtcaggcaaactata tccgggatgttcagggcgggaccgtatctccgtcatcaacagctgaagt gaccggattagcaacgcagtaacccgaaatcctctttgacaaaaacaaa gcgtgtcaggct GCGGCCGCCCATTGCTGIGGAAGCTGCCTGCACTAAT GTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTA TTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGT CGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCT GTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCA ATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTC CGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACT GCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCA TTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATA CGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATC AAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGC AACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTC ACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCT CCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCC GACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCGCG AATTGATCTG

The description of embodiments of the disclosure is not intended to be exhaustive or to limit the disclosure to the precise form disclosed. While specific embodiments of, and examples for, the disclosure are described herein for illustrative purposes, various equivalent modifications are possible within the scope of the disclosure, as those skilled in the relevant art will recognize.

For example, while method steps or functions are presented in a given order, alternative embodiments may perform functions in a different order, or functions may be performed substantially concurrently. The teachings of the disclosure provided herein can be applied to other procedures or methods as appropriate. The various embodiments described herein can be combined to provide further embodiments. Aspects of the disclosure can be modified, if necessary, to employ the compositions, functions and concepts of the above references and application to provide yet further embodiments of the disclosure. Moreover, due to biological functional equivalency considerations, some changes can be made in protein structure without affecting the biological or chemical action in kind or amount. These and other changes can be made to the disclosure in light of the detailed description. All such modifications are intended to be included within the scope of the appended claims.

Specific elements of any of the foregoing embodiments can be combined or substituted for elements in other embodiments. Furthermore, while advantages associated with certain embodiments of the disclosure have been described in the context of these embodiments, other embodiments may also exhibit such advantages, and not all embodiments need necessarily exhibit such advantages to fall within the scope of the disclosure.

The technology described herein is further illustrated by the following examples which in no way should be construed as being further limiting.

REFERENCES

Each of the references cited herein is expressly incorporated herein by reference in its entirety.

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4,968,619; 5,066,596;     5,098,998; 5,294,441; 5,330,753; 5,387,744; 5,424,065; 5,468,485;     5,527,678; 5,627,067; 5,628,996; 5,643,771; 5,654,184; 5,656,488;     5,662,905; 5,672,345; 5,679,880; 5,686,079; 5,695,983; 5,717,071;     5,731,196; 5,736,367; 5,747,028; 5,770,214; 5,773,007; 5,811,105;     5,824,538; 5,830,702; 5,837,509; 5,837,541; 5,840,483; 5,843,426;     5,855,879; 5,855,880; 5,869,066; 5,874,088; 5,877,159; 5,888,799;     6,024,961; 6,051,416; 6,077,678; 6,080,849; 6,100,388; 6,129,917;     6,130,082; 6,150,170; 6,153,203; 6,177,083; 6,190,657; 6,207,167;     6,245,338; 6,254,875; 6,284,477; 6,294,655; 6,337,072; 6,339,141;     6,365,163; 6,365,723; 6,365,726; 6,372,892; 6,383,496; 6,410,012;     6,413,523; 6,426,191; 6,444,445; 6,447,784; 6,471,964; 6,475,482;     6,495,661; 6,500,419; 6,506,550; 6,511,666; 6,531,313; 6,537,558;     6,541,623; 6,566,121; 6,593,147; 6,599,509; 6,610,300; 6,610,529;     6,653,128; 6,682,729; 6,685,935; 6,719,980; 6,737,521; 6,749,831;     6,752,994; 6,780,405; 6,825,028; 6,855,814; 6,863,894; 6,872,547;     6,887,483; 6,905,691; 6,913,753; 6,916,478; 6,923,958; 6,923,972;     6,962,696; 6,992,237; 6,994,860; 7,005,129; 7,018,835; 7,026,155;     7,045,336; 7,056,700; 7,063,850; 7,083,794; 7,094,410; 7,115,269;     7,144,580; 7,144,982; 7,183,105; 7,195,757; 7,226,588; 7,235,234;     7,264,812; 7,279,464; 7,341,725; 7,341,841; 7,341,860; 7,354,592;     7,393,525; 7,407,790; 7,425,438; 7,452,531; 7,459,161; 7,473,247;     7,510,717; 7,514,089; 7,514,415; 7,531,723; 7,541,043; 7,569,219;     7,569,552; 7,569,682; 7,588,767; 7,588,771; 7,601,804; 7,622,107;     7,625,572; 7,657,380; 7,662,398; 7,666,656; 7,691,393; 7,695,725;     7,700,091; 7,700,104; 7,718,179; 7,732,187; 7,754,221; 7,758,876;     7,763,420; 7,772,386; 7,776,527; 7,794,734; 7,803,531; 7,803,990;     7,807,184; 7,807,456; 7,820,184; 7,829,104; 7,833,775; 7,842,289;     7,842,290; 7,850,958; 7,850,970; 7,871,604; 7,871,815; 7,871,816;     7,887,816; 7,919,081; 7,927,606; 7,930,107; 7,951,386; 7,951,786;     7,955,600; 7,960,518; 7,972,604; 7,985,573; 7,993,651; 8,012,466;     8,021,662; 8,021,848; 8,034,359; 8,043,857; 8,048,428; 8,049,000;     8,053,181; 8,053,421; 8,066,987; 8,071,084; 8,071,319; 8,076,099;     8,101,396; 8,114,409; 8,114,414; 8,124,068; 8,124,408; 8,133,493;     8,137,904; 8,147,820; 8,168,421; 8,173,773; 818761.0; 8,202,516;     8,207,228; 8,211,431; 8,221,769; 8,227,584; 8,241,623; 8,241,631;     8,241,637; 8,257,713; 8,273,361; 8,287,883; 8,288,359; 8,318,661;     8,323,668; 8,323,959; 8,329,685; 8,337,832; 8,337,861; 8,343,509;     8,343,512; 8,349,586; 8,357,486; 8,357,533; 8,361,707; 8,367,055;     8,399,618; 8,440,207; 8,445,254; 8,445,426; 8,445,662; 8,460,666;     8,465,755; 8,470,551; 8,481,055; 8,501,198; 8,524,220; 8,551,497;     8,557,789; 8,568,707; 8,580,280; 8,586,022; 8,591,862; 8,609,114;     8,623,350; 8,628,776; 8,632,783; 8,633,305; 8,642,257; 8,642,656;     8,647,642; 8,658,350; 8,663,940; 8,669,355; 8,673,311; 8,679,505;     8,703,153; 8,715,929; 8,716,254; 8,716,343; 8,722,064; 8,748,150;     8,758,766; 8,771,669; 8,772,013; 8,778,683; 8,784,829; 8,784,836;     8,790,909; 8,840,908; 8,853,382; 8,859,256; 8,877,212; 8,883,147;     8,889,121; 8,889,150; 8,895,062; 8,916,372; 8,926,993; 8,937,074;     8,951,531; 8,956,618; 8,956,621; 8,956,859; 8,961,989; 8,980,279;     8,992,943; 9,005,665; 9,011,870; 9,012,213; 9,017,986; 9,023,635;     9,040,059; 9,040,233; 9,045,528; 9,045,742; 9,050,285; 9,050,319;     9,051,574; 9,056,909; 9,062,297; 9,068,187; 9,107,864; 9,140,698;     9,161,974; 9,163,219; 9,169,302; 9,173,930; 9,173,935; 9,173,936;     9,180,183; 9,181,546; 9,198,960; 9,200,251; 9,200,289; 9,205,142;     9,220,764; 9,248,177; 9,255,149; 9,255,283; 9,265,804; 9,267,108;     9,289,481; 9,297,015; 9,303,264; 9,309,493; 9,315,817; 9,320,787;     9,320,788; 9,333,251; 9,339,533; 9,358,283; 9,364,528; 9,365,625;     9,376,686; 9,408,880; 9,415,077; 9,415,098; 9,421,252; 9,428,572;     9,441,204; 9,453,227; 9,457,074; 9,457,077; 9,463,238; 9,474,831;     9,480,740; 9,481,884; 9,481,888; 9,486,513; 9,487,577; 9,492,534;     9,499,606; 9,504,750; 9,506,922; 9,526,778; 9,529,005; 9,539,313;     9,540,407; 9,546,199; 9,549,956; 9,556,442; 9,561,270; 9,562,080;     9,562,837; 9,566,321; 9,566,322; 9,567,375; 9,580,478; 9,580,718;     9,592,283; 9,593,339; 9,597,379; 9,598,697; 9,603,799; 9,610,342;     9,616,114; 9,622,486; 9,636,386; 9,642,881; 9,642,904; 9,649,345;     9,651,559; 9,655,815; 9,657,085; 9,657,327; 9,662,385; 9,663,758;     9,670,270; 9,695,229; 9,714,426; 9,717,782; 9,730,996; 9,737,592;     9,737,601; 9,739,773; 9,750,802; 9,758,572; 9,764,021; 9,775,896;     9,795,641; 9,796,762; 9,801,930; 9,808,517; 9,814,772; 9,827,305;     9,844,592; 9,845,342; 9,855,336; 9,856,311; 9,867,785; 9,872,898;     9,878,023; 9,878,024; 9,878,043; 9,884,108; 9,885,051; 9,889,165;     9,901,082; 9,907,755; 9,907,845; 9,913,893; 9,925,257; 9,950,063;     9,986,724; 9,987,355; 9,994,809; 9,999,660; 20010014673;     20020025325; 20020028215; 20020044938; 20020068068; 20020076417;     20020077272; 20020081317; 20020086032; 20020086332; 20020090376;     20020132789; 20020146430; 20020151462; 20020156009; 20020176848;     20030017162; 20030023075; 20030045492; 20030065039; 20030068328;     20030100100; 20030108562; 20030108957; 20030124516; 20030125278;     20030130827; 20030152589; 20030153527; 20030157637; 20030166099;     20030166279; 20030170211; 20030170613; 20030176377; 20030180260;     20030180304; 20030180320; 20030185802; 20030186908; 20030190601;     20030190683; 20030190749; 20030194714; 20030194755; 20030194798;     20030198995; 20030198996; 20030199005; 20030199088; 20030199089;     20030202937; 20030203411; 20030203481; 20030207833; 20030211086;     20030211103; 20030211461; 20030211476; 20030211599; 20030219408;     20030219888; 20030224369; 20030224444; 20030232335; 20030235577;     20040005700; 20040009540; 20040009936; 20040013658; 20040013689;     20040023310; 20040033539; 20040052817; 20040053209; 20040077067;     20040121307; 20040121474; 20040126871; 20040131641; 20040132678;     20040137003; 20040156865; 20040192631; 20040202663; 20040213804;     20040228877; 20040229338; 20040237147; 20040258703; 20040258707;     20040265337; 20050008618; 20050010032; 20050026866; 20050042755;     20050048076; 20050075298; 20050106151; 20050106176; 20050129711;     20050163791; 20050175630; 20050180985; 20050222057; 20050229274;     20050232937; 20050233408; 20050249706; 20050249752; 20050255125;     20050271643; 20050271683; 20050281841; 20050287123; 20060018877;     20060019239; 20060074039; 20060078572; 20060083716; 20060115494;     20060121045; 20060121054; 20060140971; 20060140975; 20060147418;     20060147461; 20060153875; 20060171960; 20060182754; 20060189792;     20060193874; 20060233835; 20060240494; 20060246083; 20060257415;     20060269570; 20070031382; 20070031458; 20070037225; 20070048331;     20070059323; 20070104733; 20070104736; 20070110717; 20070116725;     20070122881; 20070128216; 20070134214; 20070134272; 20070141082;     20070154495; 20070169226; 20070189982; 20070258901; 20070281328;     20070286874; 20070298012; 20080008718; 20080020441; 20080038296;     20080063655; 20080095794; 20080107653; 20080112974; 20080124355;     20080131466; 20080138359; 20080181892; 20080188436; 20080193373;     20080213308; 20080241179; 20080241858; 20080254058; 20080261869;     20080286852; 20080311081; 20080317742; 20090017000; 20090017048;     20090028892; 20090053186; 20090074816; 20090081250; 20090081257;     20090104204; 20090117151; 20090117152; 20090123426; 20090142310;     20090148473; 20090169517; 20090169562; 20090180987; 20090181078;     20090196887; 20090214476; 20090227013; 20090253778; 20090263414;     20090263418; 20090285844; 20090297552; 20090297561; 20090304750;     20090305398; 20090324503; 20090324576; 20090324638; 20090324641;     20100047286; 20100055082; 20100055127; 20100068214; 20100092438;     20100092518; 20100099600; 20100129406; 20100135961; 20100136048;     20100136055; 20100136058; 20100137192; 20100166786; 20100166800;     20100172938; 20100172976; 20100189691; 20100196524; 20100209446;     20100226891; 20100226931; 20100226941; 20100233212; 20100233213;     20100239546; 20100272748; 20100272759; 20100285592; 20100291148;     20100297184; 20100297740; 20100303862; 20100310602; 20100322957;     20110008389; 20110014274; 20110020401; 20110021416; 20110052628;     20110059126; 20110064723; 20110064766; 20110070290; 20110086059;     20110104186; 20110110979; 20110111481; 20110111496; 20110123565;     20110183342; 2011019509:3; 20110200631; 20110201092; 20110201676;     20110206694; 20110209228; 20110212090; 20110213129; 20110217323;     20110243992; 20110256214; 20110268739; 20110275585; 20110281330;     20110287046; 20110293662; 20110312020; 20120003298; 20120009247;     20120014881; 20120027811; 20120036589; 20120039931; 20120039994;     20120058142; 20120071545; 20120077206; 20120093773; 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What is claimed is:
 1. A live genetically engineered bacterium of genus Salmonella or species Escherichia coli, comprising a deoxyribonucleic acid sequence having an associated promoter, translated by the live genetically engineered bacterium to a fusion peptide comprising a SARS-CoV-2 receptor binding domain having SEQ ID NO. 003 fused in-frame with an adjuvant peptide encoding sequence, and having a bacterial secretion peptide sequence.
 2. The live genetically engineered bacterium according to claim 1, wherein the deoxyribonucleic nucleic acid sequence is integrated into the live genetically engineered bacterial genome.
 3. The live genetically engineered bacterium according to claim 1, wherein the live genetically engineered bacterium is E. coli.
 4. The live genetically engineered bacterium according to claim 1, wherein the live genetically engineered bacterium is Salmonella.
 5. The live genetically engineered bacterium according to claim 1, wherein the adjuvant peptide comprises a dimer of P28 having SEQ ID NO.
 007. 6. The live genetically engineered bacterium according to claim 1, wherein the adjuvant peptide is selected from the group consisting of flagellin, a flagellin toll-like receptor binding region, a dimer of C3d p28 having SEQ ID NO. 007, and a dimer of C3d p28 having SEQ ID NO. 007 with an internal flagellin peptide.
 7. The live genetically engineered bacterium according to claim 1, wherein the live genetically engineered bacterium expresses a gut colonization factor.
 8. The live genetically engineered bacterium according to claim 7, wherein the gut colonization factor is selected from the group consisting of colicin A, E1, E2, E3, E4, E5, E6, E7, E8, E9, DF13, K, N, U, B, D, Ia, and M.
 9. The live genetically engineered bacterium according to claim 1, wherein the peptide further comprises an angiotensin converting enzyme 2 binding peptide.
 10. The live genetically engineered bacterium according to claim 1, wherein the bacterial secretion signal peptide sequence is selected from the group consisting of YebF, ice nucleation protein, and HlyA.
 11. The live genetically engineered bacterium according to claim 1, wherein the live genetically engineered bacterium co-expresses both a colicin immunity peptide and a colicin lysis peptide.
 12. A live genetically engineered bacterium of genus Salmonella or species Escherichia coli, comprising a deoxyribonucleic acid sequence having a promoter and being translated by the live genetically engineered bacterium to a fusion peptide comprising: a SARS-CoV-2 spike protein receptor binding domain having SEQ ID NO. 003; an adjuvant peptide encoding sequence; and a bacterial secretion signal peptide.
 13. A method of immunizing a human against SARS-Cov-2 viral infection comprising: administering to the human a composition comprising live genetically engineered bacteria of genus Salmonella or species Escherichia coli, comprising a deoxyribonucleic acid sequence having a promoter and being translated by the live genetically engineered bacterium to a fusion peptide comprising: a SARS-CoV-2 spike protein receptor binding domain having SEQ ID NO. 003; an adjuvant peptide encoding sequence; and a bacterial secretion signal peptide; wherein the live genetically engineered bacteria colonize a gut tissue of the human; wherein the live genetically engineered bacteria secrete the fusion peptide comprising SARS-CoV-2 receptor binding domain in the gut tissue; wherein the secreted peptides induce an immune response in the human to the SARS-CoV-2 receptor binding domain; and wherein the live genetically engineered bacteria is cleared from the human.
 14. The method according to claim 13, wherein the deoxyribonucleic acid sequence is integrated into the genome of the live genetically engineered bacterium and comprises a promoter.
 15. The method according to claim 13, where the deoxyribonucleic acid sequence encodes the SARS-CoV-2 receptor binding domain fused in-frame with the adjuvant peptide encoding sequence.
 16. The method according to claim 13, wherein the live genetically engineered bacterium expresses an angiotensin converting enzyme 2 binding peptide.
 17. The method according to claim 13, wherein the deoxyribonucleic acid sequence encodes a fusion protein further comprising a peptide sequence selected from the group consisting of YebF, ice nucleation protein, and HlyA. 